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Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

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SKU
TA130003
Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]
Size:
250 ug
Conjugation:
HRP
  $380.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, ICC, WB
Recommended Dilution
  • Immunohistochemistry / Immunocytochemistry (IHC/ICC): 1:500 – 1:5,000
  • Western Blot (WB):
  • Chromogenic substrates: 1:5,000 – 1:100,000
  • Enhanced Chemiluminescence (ECL) substrates: 1:10,000 – 1:200,000
  • ELISA: 1:5,000 – 1:100,000
Reactivity Mouse IgG
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Mouse IgG, whole molecule
Buffer 1mg/ml in PBS (pH 7.2) with 10 mg/ml Bovine Serum Albumin (BSA). IgG and Protease free. Contains 0.01% (w/v) Gentamicin Sulfate as preservative.
Presentation State Liquid
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Conjugation HRP
Storage This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below.
Shipping Blue Ice
Note Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody was previously named as TA100015
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Documents
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file-alt Citations

H2S regulation of ferroptosis attenuates sepsis‑induced cardiomyopathy
Cao, Zeng, Zhao et al
Mol Med Rep (2022) 26 (5) DOI: 10.3892/mmr.2022.12851
Cited by 41
AI Summary: In this study, OriGene products—including the β-actin antibody (cat. no. TA328071), Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG (cat. no. TA130003), and Goat Anti-Rabbit IgG Secondary Antibody (cat. no. TA130015)—were used as key reagents for western blot analysis to assess protein expression related to ferroptosis in cardiomyocytes.
Snippet: …TA328071;OriGene Technologies, Inc.). …TA130003;OriGene Technologies, Inc.) and Goat Anti-Rabbit IgG Secondary Antibody (1:10,000;cat. … TA130015 OriGene Technologies, Inc.). …
WB
Insights into Increasing Selenate Reductase Enzyme Activity in the Presence of Nitrogen-Doped Graphite Electrodes for Selenium Effluent Treatment
Mohapatra, Robinson, Huang et al
Water (2022) 14 (6), 931 DOI: 10.3390/w14060931
Cited by 3
AI Summary: OriGene’s peroxidase-conjugated Goat anti-mouse IgG (TA130003) was used as a secondary antibody in immunodetection assays to analyze protein expression in the study.
Snippet: and then incubated for 1 h with both 1:1000 diluted peroxidaseconjugated Goat anti-mouse IgG (TA130003, Origene) and 1:7500 diluted Precision Protein Strep Tactin-HRP conjugate (Bio-Rad #1610380, Mississauga, ON, Canada),
Benazepril hydrochloride protects against doxorubicin cardiotoxicity by regulating the PI3K/Akt pathway
Zhan, Wang, Zhang et al
Exp Ther Med (2021) 22 (4), 1082 DOI: 10.3892/etm.2021.10516
Cited by 17
AI Summary: In this study, OriGene's HRP-conjugated secondary antibodies (anti-mouse TA130003 and anti-rabbit TA130024) were used to detect protein expression in experiments investigating the protective effects of benazepril hydrochloride against doxorubicin-induced cardiotoxicity.
Snippet: no. sc-293125) were obtained from Santa Cruz Biotechnology, Inc., and the anti-mouse (1:5,000;TA130003) or anti-rabbit (1:5,000;TA130024) HRP-conjugated secondary antibodies were supplied by OriGene Technologies, Inc
FigureFigureFigure
WB
Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells
Shi, Fu, Wang
Oncol Lett (2021) 22 (1), 527 DOI: 10.3892/ol.2021.12788
Cited by 5
AI Summary: In this study, the HRP-conjugated secondary antibody (cat. no. TA130003) from OriGene Technologies, Inc. was used for western blotting to detect protein expression levels, contributing to the analysis of GLUT3’s role in STYK1/NOK-mediated metabolic reprogramming and cell proliferation.
Snippet: no. 2146), both from Cell Signaling Technology, Inc.;and HRP-conjugated secondary antibody (cat. no. TA130003) from OriGene Technologies, Inc. MTT, DMSO and G418 were purchased from Sigma-Aldrich;Merck KGaA. Plasmid construction and transient transfection
FigureFigureFigure
WB
Signature of high altitude adaptation in the gluteus proteome of the yak
Xin, Chai, Zhang et al
J. Exp. Zool. B Mol. Dev. Evol. (2020)
Cited by 17
AI Summary: OriGene products, specifically the TA130003 and TA140003 HRP-conjugated goat anti-rabbit IgG secondary antibodies, were used to detect target proteins in the gluteus muscle samples, enabling the analysis of protein expression differences related to high altitude adaptation in yaks.
Snippet: TA130003;OriGene Technologies, Inc.) or HRP‑conjugated goat anti‑rabbit IgG (1:10,000;cat no. TA140003;OriGene Technologies, Inc.) secondary antibodies for 2 h at room temperature, and washed three times with TBST solution
Anti-tumor peptide SA12 inhibits metastasis of MDA-MB-231 and MCF-7 breast cancer cells via increasing expression of the tumor metastasis suppressor genes, CDH1, nm23-H1 and BRMS1
Yang, Lin, Gao et al
Exp Ther Med (2020)
Cited by 3
AI Summary: OriGene products, including the anti-nm23-H1 antibody (cat. no. TA328071), HRP-labelled goat anti-mouse secondary antibody (cat. no. TA130003), and HRP-labelled goat anti-rabbit secondary antibody (cat. no. TA140003), were used for detecting protein expression levels in the study.
Snippet: no. TA328071), the horse‑ radish (HRP)‑labelled goat anti‑mouse secondary antibody (cat. no. TA130003) and the HRP‑labelled goat anti‑rabbit secondary antibody (cat. no. TA140003) were purchased from OriGene Technologies, Inc. Cell lines and culture
Showing 1-6 of 29 papers.
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