Secondary Antibodies

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592579 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 4 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4) with TA592587 at 1:2000.

Immunoblot analysis of 7 different lysates (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3;4.Human IgG4; 5.Human IgG F(ab)'2; 6.human Kappa chain constant region; 7.Human Lambda chain constant region) with TA592621 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592637 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592642 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592580 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 5 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4; 5.Native Human IgG F(ab)'2 ) with TA592626 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592577 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592582 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592572 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592576 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 4 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4) with TA592587 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592572 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592576 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592579 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 5 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4; 5.Native Human IgG F(ab)'2 ) with TA592626 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592642 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592577 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592580 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592582 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 7 different lysates (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3;4.Human IgG4; 5.Human IgG F(ab)'2; 6.human Kappa chain constant region; 7.Human Lambda chain constant region) with TA592621 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592637 at 1:2000. The 8 lysates came from different sources.

Typical experiment image where the complexes Ag–AbI–AbII are attached to the nanoparticles trapped in the magnetic traps and the fluorogenic substrate is flowed at different rates, where the measurement areas selected for the fluorescence analysis are shown. Measurement with a flow rate of (a) 1 μl/h, (b) 2 μl/h, (c) 5 μl/h, and (d) 10 μl/h. Experiment with anti-rabbit IgG labeled alkaline phosphatase was used (p/n R1364AP) and an anti-biotin rabbit IgG (p/n 100-4198) AbI concentration of 100 pg/ml. FIG. 6. PMID: 32038740.

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody: Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin Antibody at 1/1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT . Predicted/Observed size: 42 kDa.

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n R1364P). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n R1364P) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Immunoblot analysis of 5 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4; 5.Native Human IgG F(ab)'2 ) with TA592626 at 1:2000.

ELISA results of purified Sheep anti-Mouse IgG tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Dky-anti-Sheep HRP conjugate (p/n 613-7302), and TMB substrate (p/n TMBE-1000).

ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation tested against purified Mouse IgG2b HRP. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The working dilution is 1:82,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody, clone W5/25 (MCA55R) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. Low power.

Immunofluorescence of Goat Anti-Rabbit IgG (H&L) Antibody Fluorescein Conjugated. Tissue: oyster visceral mass tissue. Fixation: 4% paraformaldehyde overnight. Primary Antibody: Unstained samples (left column); CBD-SNAP antibody (middle column); or CBD-SNAP and SNAP-tag double-stained sections with rabbit anti-SNAP antibody (right column) diluted 1:200 in PBST with 1% BSA overnight at 4°C. Secondary Antibody: positive control Anti-Rabbit Alexa 488 (top row) or goat anti-rabbit IgG (heavy & light chain) antibody (FITC) (bottom row) diluted 1:400 in PBST with 1% BSA for 2h at °C. Counterstain: DAPI for 15min at RT. Independently Validated by antibodies-online GmbH (p/n ABIN7539591/ ABIN101988) courtesy of Clemson University.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592579 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 4 different native protein (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3; 4.Human IgG4) with TA592587 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592642 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592580 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592582 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of 7 different lysates (Lane 1.Human IgG1; 2.Human IgG2; 3.Human IgG3;4.Human IgG4; 5.Human IgG F(ab)'2; 6.human Kappa chain constant region; 7.Human Lambda chain constant region) with TA592621 at 1:2000.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592572 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592577 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype (IgG1, IgG2a, IgG2b, IgG3) with TA592637 at 1:2000. The 8 lysates came from different sources.

Immunoblot analysis of randomly picked 8 different lysates containing 4 different mouse IgG isotype(IgG1, IgG2a, IgG2b, IgG3) with TA592576 at 1:2000. The 8 lysates came from different sources.

Dot Blot results of Goat Fab Anti-Mouse IgG Antibody. Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Goat Fab Anti-Mouse IgG at 1µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30min at RT. Imaged with BioRad ChemiDoc, Chemi filter.

Western Blot of Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size) and blocked with 5% BLOTTO (p/n B501-0500) o/n at 4°C. The membrane was then probed with a 1/2,500 dilution of HRP Rabbit anti-Mouse IgG L - Cat# H R1253HRP for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used.

ELISA Results of purified Goat Anti-Human IgM Peroxidase Conjugated Antibody tested against purified Human IgM. The working dilution for Anti-Human IgM HRP is 1:120,000. Each well was coated in duplicate with 1.0 µg of Human IgM (p/n 009-0107). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and TMBE-1000 substrate.