Mouse IgG (Fc specific) Goat Polyclonal Antibody
| Product Data | |
| Application | ELISA, IF, WB |
|---|---|
| Recommended Dilution | This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. FLISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000. Immunofluorescence: 1/1,000-1/5,000. |
| Reactivity | Mouse |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Mouse IgG F(c) fragment. |
| Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab’)2. |
| Buffer | 0.01M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: FITC State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free) Preservative: 0.01% (w/v) Thimerisol Label: Fluorescein Absorption emission: 495 / 528 nm Molar radio: 3.1 |
| Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Purification | Immunoaffinity Chromatography |
| Conjugation | FITC |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Shipping | Ambient |
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