Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody

SKU R1454HRP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Product Data

• Application: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. ; Recommended Dilutions: ; ELISA: 1/20,000-1/100,000. ; Western Blot: 1/2,000-1/10,000.; Immunohistochemistry: 1/1,000-1/5,000.; Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product.
• Reactivity: Rabbit
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.; This antibody has been cross adsorbed and no reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Buffer: 0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; Label: HRP; State: Lyophilized purified Ig fraction; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Gentamicin Sulfate; Label: Horseradish Peroxidase ; Conjugation Chemistry: Periodate
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Shipping: Ambient