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Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]

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SKU
AM20252HR-N
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP
Size:
200 ug
Conjugation:
HRP Biotin FITC Unconjugated TRITC
  $995.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name NI 132 (HP 6186)
Application ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000.
Histochemical Use: 1/5-1/20.
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgG1 isolated from pooled Human serum.
Specificity The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct Binding Enzyme Immunoassay, Immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer PBS, pH 7.2 without preservatives or foreign proteins
Label: HRP
State: Lyophilized purified Ig fraction
Label: Horseradish Peroxidase enriched for Isoenzyme C (RZ=3.2)
Molar radio: Peroxidase/IgG ~2.2
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation HRP
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
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Documents
Resources

file-alt Citations

Significance of low level infliximab in the absence of anti-infliximab antibodies
Ungar, Anafy, Yanai et al
World J Gastroenterol (2015) 21 (6), 1907-14 DOI: 10.3748/wjg.v21.i6.1907
Cited by 19
AI Summary: In this study, OriGene's anti-human lambda chain antibody (Cat# TA500029) was used in the anti-lambda ELISA to detect antibodies to infliximab, contributing to the evaluation of double negative sera in inflammatory bowel disease patients treated with infliximab.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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