Quantify exosome subpopulations — straight from biofluid

Direct, quantitative detection of exosome subpopulations from serum, plasma, and cell culture media. No ultracentrifuge, no upstream isolation — just sample in, particle counts out.

CD9⁺ · CD81⁺ Serum · Plasma · CCM High sensitivity · intact-EV specific Matrix validated

The kits

Two tetraspanin-defined subpopulation assays

Run either kit alone, or both together to profile complementary CD9⁺ and CD81⁺ exosome subsets from the same sample set.

CD9 detection SKU · EA200081

Human Exosome ELISA Kit (CD9⁺)

Quantifies CD9-positive exosomes directly from native biofluids
— no separate isolation step, minimizing EV loss across the workflow.     

  • Particle-based quantification of CD9⁺ subpopulation
  • Sensitivity ≈ 1.91 × 10⁶ particles/mL (LLOD)
  • Validated in serum, EDTA plasma & cell culture media
  • Lyophilized, pure, NTA-characterized LNCaP-derived exosome control standard
CD81 detection SKU · EA200082

Human Exosome ELISA Kit (CD81⁺)

Quantitative sandwich ELISA for CD81⁺ exosomes in cell culture supernatants, serum, plasma, and purified EV preparations — for characterization, biomarker studies, and QC of EV isolation methods.

  • Detects intact CD81⁺ vesicles, not free CD81 protein
  • Sensitivity ≈ 4.67 × 10⁶ particles/mL (LLOD)
  • Serum, plasma, cell culture media & purified EVs
  • Lyophilized, pure, NTA-characterized MCF-7-derived exosome control standard

Coming soon Expanding subpopulation detection — EpCAM⁺, NCAM1⁺, and L1CAM⁺ exosome ELISA kits for tumor- and neuron-derived EV studies.

Performance & application data

Validated data for both kits

Each kit is calibrated with an 8-point LNCaP- or MCF-7–derived exosome standard curve and characterized for sensitivity, recovery, and reproducibility. Select a kit to view its data.

1.91 × 10⁶
particles/mL
Sensitivity (LLOD) — ≈ 13.2 ng/mL total exosome protein equivalent
R² = 0.9935
  
Standard-curve linearity across the quantitative range
4.53 × 10⁶ – 2.9 × 10⁸
particles/mL
Quantitative assay range (LNCaP EV standard)
CD9 standard curve
CD9 standard curve (LNCaP EV standard). Annotated points: human EDTA plasma (1:50), human serum (1:50), and MCF-7 cell-culture media (1:100).
Spike recovery
Cell culture media 95%
Human plasma 109%
Human serum 96%
4.67 × 10⁶
particles/mL
Sensitivity (LLOD) — ≈ 29.19 ng/mL total exosome protein equivalent
3.13 × 10⁶ – 2.0 × 10⁸
particles/mL
8-point standard range (MCF-7 EV, 2× serial dilution)
≤ 5.6% / ≤ 5.1%
Intra-assay / inter-assay precision (CV)
CD81 standard curve
CD81 standard curve (MCF-7 EV standard). Example interpolated values — plasma 3.93 × 10⁶, cell-culture media 1.26 × 10⁸, serum 2.50 × 10⁶ particles/mL.
Spike recovery
Cell culture media 97.4%
Human plasma 89.4%
Human serum 93.7%
Intact-particle specific — detects intact exosomes, not free CD81; denatured (boiled) exosomal protein is not recognized (R² = 0.9991, intact EV).

Both kits: sandwich ELISA, OD450 readout, ~3–5 hr assay (capture step extendable overnight at 4 °C). Linearity on dilution within ~90–110% of expected across plasma, serum, and cell-culture media.

Answers

Exosome ELISA FAQs