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Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]

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SKU
AM20249AF-N
Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites
Size:
500 ug
Conjugation:
Unconjugated
  $680.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name NI 69 (A89-034) and NI 184 (A89-035)
Application ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgA isolated from pooled Human serum
Specificity The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA.
It reacts with both subclasses of IgA as tested in Haemagglutination, Haemagglutination inhibition, indirect binding Enzyme Immunoassay, competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996). J. Immunol. Methods 193, 103-148).
Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer State: Ascites
State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without preservatives or foreign proteins.
Presentation State Ascites
Reconstitution Method Restore by adding 0.5 ml sterile distilled water.
Concentration 1.0 mg/ml
Conjugation Unconjugated
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Avoid repeated freezing and thawing.
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Documents
Resources

file-alt Citations

Pseudomonas aeruginosa Affects Airway Epithelial Response and Barrier Function During Rhinovirus Infection
Endres, Hügel, Boland et al
Front Cell Infect Microbiol (2022) 12, 846828 DOI: 10.3389/fcimb.2022.846828
Cited by 14
AI Summary: OriGene products contributed to this study by providing recombinant human IL-6 (Cat# TP303703) and anti-FOXJ1 antibody (Cat# TA500648), which were used to analyze cytokine degradation and epithelial cell differentiation, respectively.
FigureFigureFigure
Effects of a lactulose-rich diet on fecal microbiome and metabolome in pregnant mice
Zhang, Chen, Zhao et al
J. Agric. Food Chem. (2019)
AI Summary: OriGene’s monoclonal antibodies against CD4+, CD8+, and IgA were used for immunohistochemistry to detect the expression of these immune markers in the mouse colonic mucosal epithelium in this study.
Snippet: 135 (IHC) staining. Monoclonal antibodies of CD4+, CD8+, and IgA (1:100, OriGene 136 Technologies, Inc, Rockville, MD, USA) were used for the IHC to detect the expressions of 137 CD4+ and CD8+ lymphocytes and IgA in the mouse colonic mucosal epithelium
IHC
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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