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Mouse IgG (H+L chain) Sheep Polyclonal Antibody

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SKU
R1256P
Sheep Anti-Mouse IgG (H&L) Antibody, 2 mg
Size:
2 mg
Conjugation:
Unconjugated Biotin FITC HRP
  $325.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:100,000
Reactivity Mouse
Antibody Host Sheep
Clonality Polyclonal
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in sheep.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Mouse IgG and Mouse Serum.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation Unconjugated
Storage Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Shipping Blue Ice
Note Anti-Mouse IgG antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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Documents
Resources

file-alt Citations

Ergometrine sensing in rye flour by a magnetic bead-based immunoassay followed by flow injection analysis with amperometric detection
Höfs, Jaut, Schneider
Talanta (2023) 254, 124172 DOI: 10.1016/j.talanta.2022.124172
Cited by 15
AI Summary: In this study, OriGene's recombinant mouse monoclonal anti-ergometrine antibody (Cat# AM09306PU-N) was used as a key reagent in the development of the magnetic bead-based immunoassay for sensitive detection of ergometrine in rye flour.
Snippet: A certain group of mycotoxins, the ergot alkaloids, has caused countless deaths throughout human history. They are found in rye and other cereals and ingesting Save;H (Berlin, Germany). Polyclonal sheep anti-mouse IgG antibodies IgG (R1256P) were purchased from Acris Antibody GmbH (Herford, Germany). For ELISA, transparent high-binding 96-well microplates (polystyrene) from Greiner Bio-One (S
ELISA
Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’‐Tetramethylbenzidine in Amperometric Assays
Höfs, Hülagü, Bennet et al
ChemElectroChem (2021) 8 (13), 2597-2606 DOI: 10.1002/celc.202100446
Cited by 18
AI Summary: In this paper, OriGene's Mouse monoclonal anti-Ochratoxin A antibody (Cat# AM10042PU-N) was used as the primary antibody in the immunomagnetic assay, enabling the specific detection of ochratoxin A in beer samples.
Snippet: Secondary polyclonal sheep anti-mouse IgG antibodies IgG (R1256P) were obtained from Acris Antibody GmbH, (Herford, Germany). Potassium hexacyanoferrate(III), 98+% (K3[Fe(CN)6]) and potassium chloride, ACS, 99.0-100.5% from Alfa Aesar Co.Inc
ELISA
Development of immunoassays for the rapid determination of the endocrine disruptor bisphenol A released from polymer materials and products
Raysyan
Thesis (2021)
Snippet: … This simple technology can significantly reduce the uncertainty in the various and sometimes highly controversial statistical figures of a . , like Save;HRP-conjugated sheep anti-mouse-IgG (R1256HRP) and sheep anti-mouse IgG (R1256P) provided by Acris Antibody GmbH (Herford, Germany)
ELISA
Zeolitic Imidazolate Framework Antibody Conjugates in Bioanalytical Applications
Chapartegui Arias, Raysyan, Belenguer et al
ChemRxiv (2020)
Snippet: Anti-mouse IgG from sheep (R1256P), 2 mg/ml, in PBS buffer, was purchased from Acris Antibody GmbH (Herford, Germany). Mouse anti-CBZ IgG (AK2512/01 ), 1.75 mg/mL, in PBS buffer, ordering code BAM-mab 01 (CBZ) was obtained from BAM.[1] 1.7 Solvent for;- Anti-mouse IgG from sheep (R1256P), 2 mg/ml, in PBS buffer, was purchased from Acris Antibody GmbH (Herford, Germany). …
ELISA
Microfluidic Electrochemical Immunosensor for the Trace Analysis of Cocaine in Water and Body Fluids
Abdelshafi, Bell, Rurack et al
Drug Test Anal (2018)
AI Summary: OriGene products, specifically the monoclonal anti-cocaine antibody (cat. no. MAB4029) and polyclonal anti-mouse IgG from sheep (cat. no. R1256P), were used as key reagents for the immunoassay development in this study's microfluidic electrochemical sensor for cocaine detection.
Snippet: cat. no. MAB4029, 3.75 mg/mL), and polyclonal anti-mouse IgG from sheep (cat. no. R1256P, 2.2 mg/mL) were purchased from OriGene (Rockville, MD). Cocaine hydrochloride (Ph Eur, BP, USP, cryst., extra pure, cat. n° 1.02562
ELISA
Screening for cocaine on Euro banknotes by a highly sensitive enzyme immunoassay
Abdelshafi, Panne, Schneider
Talanta (2017) 165, 619-624 DOI: 10.1016/j.talanta.2017.01.004
Cited by 16
AI Summary: OriGene products contributed to this study by providing the anti-cocaine monoclonal antibody (Cat# AM09316PU-N), which was essential for developing the highly sensitive enzyme immunoassay used to detect cocaine on Euro banknotes.
Showing 1-6 of 9 papers.
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