Plasmid Reconstitution Protocol
All OriGene plasmids are delivered as lyophilized plasmids and must be reconstituted before use. Read this short protocol or watch this video to learn more.
Step 1, Add Sterile Water: Carefully open the tube and add 100 ul dH2O to produce a concentration of 100 ng/ul.
Note: Dissolving the plasmid DNA in a lower volume is not recommended as an increased EDTA concentration may affect some downstream applications.
Step 2, Incubate: Close the tube and let it sit for 10 minutes at room temperature, or 4°C overnight.
Step 3, Vortex: Vortex the tube briefly and then do a quick spin to concentrate the liquid at the bottom.
The DNA is ready for immediate use in:
- Transfection into mammalian cells
- Restriction enzyme digestion
- Protein expression in cell-free systems
- PCR amplification
- Probe labeling
- E. coli transformation for DNA amplification
- Sequencing reactions