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pCas-Scramble-EF1a-GFP

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SKU
GE100021
A scrambled sequence in pCas-Guide-EF1a-GFP vector (10 µg)
  $670.00
In Stock*

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TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
500 pmol
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Specifications
Specifications
Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • Negative control vector in CRISPR/Cas9 system
  • All-in-one vector
    1. Expresses human codon-optimized Cas9
    2. Expresses a scrambled guide RNA (gRNA)
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping Ambient
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Documents
Resources

file-alt Citations

SPINK1-COL18A1 Crosstalk Shapes Epigenome and Drives Cancer Stemness in Pancreatic Ductal Adenocarcinoma
Tang, Sailo, Shang et al
bioRxiv (2025)
Cited by 1
AI Summary: OriGene products, specifically the SPINK1 Human Gene Knockout Kit (CRISPR) (Cat#KN406574) and pCas-Scramble-EF1a-GFP (Cat#GE100021), were used to manipulate SPINK1 gene expression, enabling the study of its role in regulating epigenomic states and cancer stemness in pancreatic ductal adenocarcinoma cells.
Snippet: Discovery ChromoMap DAB detection kit Ventana Cat#760-159 SPINK1 Human Gene Knockout Kit (CRISPR) Origene Cat#KN406574 pCas-Scramble-EF1a-GFP Origene Cat#GE100021
Glycosaminoglycan modifications of betaglycan regulate ectodomain shedding to fine-tune TGF-β signaling responses in ovarian cancer
Choi, Jenkins-Lane, Barton et al
Cell Commun Signal (2024) 22 (1), 128 DOI: 10.1186/s12964-024-01496-y
Cited by 7
AI Summary: OriGene products contributed to this study by providing the siRNA-27 kit (SR304839) for TIMP3 knockdown and the CRISPR/CAS9 genome-wide knockout kit (GE100021) to generate betaglycan knockout cell lines, enabling the authors to investigate the molecular mechanisms regulating TGF-β signaling in ovarian cancer.
Snippet: Origene siRNA-27 kit was used for transient knockdown of TIMP3 expression (SR304839 Locus IF 7078). Cells were reverse-transfected using Lipofectamine-RNAiMAX;the flow cytometry core at the University of South Carolin Flow Cytometry Core Facility or UAB Flow Cytometry and Single-Cell Core facility. Betaglycan CRISPR knockout cell line was generated using Origene CRISPR/CAS9 genome-wide knockout kit (GE100021). 10 MOI of CRISPR BG pCAS guide virus and 8µg/mL of polybrene were used for each infection. …
FigureFigureFigure
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation
Eng, Reed, Kokolus et al
Nat Commun (2015) 6, 6426 DOI: 10.1038/ncomms7426
Cited by 157
AI Summary: OriGene's shADRB2 and scramble constructs were used to selectively knock down β2-adrenergic receptor expression in MIA PaCa-2 cells, enabling the study of β2-adrenergic signaling in tumour response to stress and therapy.
Snippet: Colonies were isolated and then screened for luminescence activity using D-luciferin (Gold Bio) and read on a Biotek HT plate reader. shADRB2 and scramble constructs were purchased from Origene and transfected into MIA PaCa-2 cells using Fugene HD (Promega);these target sequences are specific for ​ADRB2 and do not cross-react with ​ADRB1 or ​ADRB3
Showing 1-3 of 3 papers.
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