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p-xCas9-Guide CRISPR Vector

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SKU
GE100078
p-xCas9-Guide vector (with xCas9 expression) for genomic target sequence cloning (10 µg)
  $670.00
In Stock*

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Specifications
Specifications
Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • All-in-one vector
    1. Expresses human codon-optimized xCas9
    2. BamH I and BsmB I can be used for genomic target sequence cloning
    3. Expresses gRNA containing the inserted target sequence and the gRNA scaffold
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Encapsulating Cas9 into extracellular vesicles by protein myristoylation
Whitley, Kim, Lou et al
J Extracell Vesicles (2022) 11 (4), e12196 DOI: 10.1002/jev2.12196
Cited by 62
AI Summary: OriGene’s CRISPR vectors were used as PCR templates to construct plasmids expressing N-myristoylated Cas9, enabling the study’s development of Cas9 encapsulation into extracellular vesicles for genome editing.
Snippet: in EV‐based genome editing therapy. 2 MATERIALS AND METHODS 2.1 Plasmid constructs To create non‐lentiviral vectors expressing N‐myristoylated Cas9 (mCas9), Cas9/sgRNA‐empty, CRISPR vectors (OriGene, Rockville, MD, USA) were used as PCR templates. The Src(WT;8 a.a) (forward primer) and mCas9 primer (reverse primer) (Table S1) were used to obtain a PCR product, which fused the DNA sequenc
FigureFigureFigure
Showing 1-1 of 1 papers.
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