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Annealing Buffer

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SKU
GE100007
Oligo annealing buffer (10X)
  $95.00
In Stock*

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Specifications
Specifications
Product Data
Storage Store at +4°C.
Stability Stable for a minimum of one year from date of receipt when stored at 4°C. 1X working solution is stable for up to 4 weeks when stored at 4°C.
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Modulation of SLFN11 induces changes in DNA Damage response in breast cancer
Raynaud, Ahmed, Jabeen et al
Cancer Cell Int (2023) 23 (1), 291 DOI: 10.1186/s12935-023-03144-w
Cited by 13
AI Summary: OriGene's Annealing Buffer (GE100007) was used to anneal guide RNA oligos for CRISPR-dCas9-mediated modulation of SLFN11 expression in breast cancer cell lines, facilitating the study's investigation of SLFN11's role in DNA damage response and chemosensitivity.
Snippet: μl of annealing buffer (Origene, #GE100007) with 2 μl of each oligo (100 μM) and annealed in the thermal cycler at 95 C for 4 min followed by cooling to 25 C with 1 C/ Save Cached;ion enzyme (New England Biolabs, #R0580L) as indicated in [14]. Briefly, gRNAs were obtained from Integrated DNA technology as two single strand oligos and annealed in 40  μl of annealing buffer (Origene, #GE100007) with 2  μl of each oligo (100 μM) and annealed in the thermal cycler at 95  °C for 4 min followed by cooling to 25  °C with 1 °C/minute ramp. …
FigureFigureFigure
Modulation of SLFN11 induces changes in DNA Damage response
Raynaud, Ahmed, Jabeen et al
bioRxiv (2023)
Cited by 1
Snippet: Briefly, gRNAs were obtained from Integrated DNA technology as two single strand oligos and annealed in 40 μl of annealing buffer (Origene, #GE100007) with 2 μl of
Virosecurinine induces apoptosis in human leukemia THP-1 cells and other underlying molecular mechanisms
Zhang, Gao, Zeng et al
Oncol Lett (2018) 15 (1), 849-854 DOI: 10.3892/ol.2017.7437
Cited by 10
AI Summary: OriGene contributed to this study by providing the specific primers for PI3K (NM_006218), AKT (NM_005163), mTOR (NM_004958), and PTEN (NM_000314) used in the RT-qPCR experiments to analyze gene expression changes in THP-1 cells treated with virosecurinine.
FigureFigureFigure
An Efficient Targeted Mutagenesis System Using CRISPR/Cas in Monocotyledons
Liang, Zong, Gao
Curr Protoc Plant Biol (2016) 1 (2), 329-344 DOI: 10.1002/cppb.20021
Cited by 12
Identification of miRNAs that modulate glucocerebrosidase activity in Gaucher disease cells
Siebert, Westbroek, Chen et al
RNA Biol (2014) 11 (10), 1291-300 DOI: 10.1080/15476286.2014.996085
Cited by 40
AI Summary: OriGene’s pMirTarget vector was used to clone the SCARB2 3'UTR for luciferase assays, enabling the study of miR-127-5p’s regulatory effects on LIMP-2 expression in the investigation of miRNA modulation of glucocerebrosidase activity in Gaucher disease cells.
Snippet: antibody as described above. SCARB2 luciferase assay. The full-length of the SCARB2 3'UTR covering two predicted target sites of miR-127-5p was cloned into the pMirTarget vector (Origene). For luciferase assays, HEK293 ... Related articles All 3 versions Cite SaveSaving
Genome editing in rice and wheat using the CRISPR/Cas system
Shan, Wang, Li et al
Nat Protoc (2014) 9 (10), 2395-410 DOI: 10.1038/nprot.2014.157
Cited by 797
AI Summary: OriGene's Annealing buffer, 10× (cat. no. GE100007) was used in the protocol for preparing reagents necessary for CRISPR/Cas-mediated genome editing in rice and wheat.
Snippet: Annealing buffer, 10× (OriGene, cat. no. GE100007)
Showing 1-6 of 7 papers.
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