Gene Expression
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qSTAR qPCR primer pairs against Homo sapiens gene GAPDHComponents 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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Oligo annealing buffer (10X)
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qSTAR qPCR primer pairs against Mus musculus gene TnfComponents 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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Cre recombinase mammalian expression vector
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PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
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Forward primer to sequence targets cloned in expression vectors.Applicable Vectors PS100001, PS100002, PS100003, PS100004, PS100005, PS100006, PS100007, PS100008, PS100009, PS100010, PS100011, PS100012, PS100013, PS100014, PS100016, PS100017, PS100018, PS100020, PS100022, PS100024, PS100025, PS100026, PS100027, PS100034, PS100036, PS100038, PS100039, PS100040, PS100041, PS100042, PS100043, PS100044, PS100053, PS100054, PS100057, PS100058, PS100059, PS100064, PS100065, PS100066, PS100067, PS100069, PS100071, PS100074, PS100081, PS100082, PS100088, PS100092, PS100093, PS100094, PS100101, PS100102, PS100103, PS100104, PS100105, PS100107, PS100108, PS100109, PS100110, PS100111, PS100126, PS100135, PS100136, PS100139
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CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
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Cas9 in vitro digestion buffer, 10x
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qSTAR qPCR primer pairs against Homo sapiens gene DSPPComponents 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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qSTAR qPCR primer pairs against Mus musculus gene Il18Components 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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SensiMix SYBR Master Mix - No-ROX (2x), 400 x 25 uL rxn (5ml)
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SensiFast SYBR Lo-ROX (2x), 100 x 20 uL rxn (1mL)
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Reverse primer to sequence targets cloned in expression vectors.Applicable Vectors PS100064
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qSTAR qPCR primer pairs against Mus musculus gene Spp1Components 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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SensiFast SYBR Lo-ROX (2x), 1000 x 20 uL rxn (10mL)
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Forward primer to sequence targets cloned in expression vectors.Applicable Vectors PS100030
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SensiMix SYBR Master Mix - No-ROX (2x), 100 x 25 uL rxn (1.25ml)
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Forward primer to sequence targets cloned in expression vectors.Applicable Vectors PS100002
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Forward primer to sequence targets cloned in expression vectors.Applicable Vectors PS100048
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Reverse primer to sequence targets cloned in expression vectors.