pT7-Guide-IVT

SKU
GE100025
pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)
$450.00
In Stock*
Specifications
Product Data
Function CRISPR/Cas9 T7 Vectors
Features

T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.