pT7-Cas9

CAT#: GE100014

pT7-Cas9 vector in which Cas9 is under T7 for IVT to produce Cas9 mRNA


Reconstitution Protocol

USD 650.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function CRISPR/Cas9 T7 Vectors
Features
  • T7 driven Cas9 expression vector
    1. Cas9 mRNA can be produced via in vitro transcription.
    2. For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven gRNA can be produced from a separate vector, SKU GE100025.
Disclaimer These products are manufactured and supplied by OriGene under license from ERS.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.