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pT7-Cas9

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SKU
GE100014
pT7-Cas9 vector in which Cas9 is under T7 for IVT to produce Cas9 mRNA
  $670.00
In Stock*

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Specifications
Specifications
Product Data
Function CRISPR/Cas9 T7 Vectors
Features
  • T7 driven Cas9 expression vector
    1. Cas9 mRNA can be produced via in vitro transcription.
    2. For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven gRNA can be produced from a separate vector, SKU GE100025.
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse
Pineault, Novoa, Lozovska et al
MethodsX (2019) 6, 2088-2100 DOI: 10.1016/j.mex.2019.09.003
Cited by 8
AI Summary: In this paper, OriGene’s pUC57-Kan plasmid was used as a backbone for generating long single-stranded DNA (lssDNA) templates essential for CRISPR/Cas9-mediated genome editing in mice.
FigureFigureFigure
PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction
Zhou, Ma, Sun et al
Hum. Mol. Genet. (2018)
AI Summary: OriGene’s pT7-Guide-IVT vector and pT7-Cas9 vector were used to generate CRISPR-Cas9 constructs and produce Cas9 mRNA, enabling the creation of isogenic human stem cell lines harboring PD-associated CHCHD2 mutations in this study.
Snippet: Oligos were annealed and ligated with pre-digested pT7-Guide-IVT vector (Origene), followed with in vitro mRNA. Capped and polyadenylated Cas9 mRNA was obtained from pT7-Cas9 vector (Origene) by mMESSAGE Mmachine T7 ULTRA kit (Thermo fisher scientific
Fine Tuning of Histone Demethylase KDM6A/B Improves the Development of Nuclear Transfer Embryo
Yang, Song, Liu et al
bioRxiv (2018)
Snippet: The coding region of KKDM6A and KDM6B was amplified from mouse tail tip genome. 527 Forward and reverse primers contained T7 promoter and HA sequences, respectively. To 528 prepare mRNAs for microinjection, pT7-Cas9 (OriGene, GE100014), KKDM6A and KDM6B
KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system
Yang, Song, Liu et al
EMBO reports (2018)
AI Summary: OriGene's pT7-Cas9 (GE100014) expression vector was used to prepare mRNA for microinjection, facilitating the study of KDM6A and KDM6B roles in nuclear transfer embryos.
Snippet: egion of KDM6A and KDM6B was amplified from mouse tail‐tip genome. Forward and reverse primers contained T7 promoter and HA sequences, respectively. To prepare mRNAs for microinjection, pT7‐Cas9 (OriGene, GE100014), KKDM6A and KDM6B expression vectors were linearized and subjected to phenol–chloroform extraction and ethanol precipitation. The mRNA synthesized with the mMESSAGE‐mMACHINE T7
Showing 1-4 of 4 papers.
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