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pCMV6-Entry-Cre

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SKU
GE100017
Cre recombinase mammalian expression vector
  $471.00
In Stock*

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TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Specifications
Specifications
Product Data
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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FL118, acting as a 'molecular glue degrader', binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c-Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy
Ling, Wu, Aljahdali et al
Clin Transl Med (2022) 12 (5), e881 DOI: 10.1002/ctm2.881
Cited by 40
AI Summary: OriGene products, specifically the Flag-DDX5 expression vector (RC200371) and pCMV6-Entry-Myc-DDK-DDX5 and pCMV6-Entry-Myc-DDK-Top1 vectors, were used to overexpress DDX5 and Top1 in cells, enabling the authors to investigate the role of DDX5 in regulating oncogenic protein expression and the mechanism of FL118 action in cancer models.
Snippet: Forced expression of Flag‐DDX5 increases the expression of survivin, Mcl‐1 XIAP, cIAP2 and c‐Myc. Cells were transfected with empty vector (control) or Flag‐DDX5 expression vectors (RC200371, OriGene) using Lipofectamine 2000. … Overexpressed Flag‐DDX5 was detected using t;- pCMV6-Entry-Myc-DDK-DDX5 and pCMV6-Entry-Myc-DDK-Top1 expression vectors were purchased from OriGene. …
PRMT7 deficiency enhances adipogenesis through modulation of C/EBP-β
Leem, Bae, Jeong et al
Biochem. Biophys. Res. Commun. (2019) 517 (3), 484-490 DOI: 10.1016/j.bbrc.2019.07.096
AI Summary: OriGene's pCMV6-Entry-C/EBP-β-Myc/DDK plasmid was used in this study to overexpress C/EBP-β, enabling the authors to investigate the role of PRMT7 in regulating adipogenesis through modulation of C/EBP-β activity.
Snippet: pcDNA-HA-PRMT7 and/or pCMV6-Entry-C/EBP-β-Myc/DDK (Origene) were transfected by using Lipofectamine 2000 (Invitrogen) according to manufacturer's instructions. 2.2. Total RNA preparation and real-time qRT-PCR;3T3-L1 cells were cultured in DMEM with 10% calf serum and penicillin/streptomycin. MEFs and 293T cells were cultured in DMEM with 10% fetal bovine serum (FBS) and penicillin/streptomycin. Fo...
Oncogenic KSHV-encoded interferon regulatory factor upregulates HMGB2 and CMPK1 expression to promote cell invasion by disrupting a complex lncRNA-OIP5-AS1/miR-218-5p network
Li, Wang, Feng et al
PLoS Pathog. (2019) 15 (1), e1007578 DOI: 10.1371/journal.ppat.1007578
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry-C-Myc-p53 and pCMV6-Entry-C-Myc expression constructs, which were used to investigate the roles of p53 and related proteins in KSHV-induced cell invasion mechanisms.
Snippet: The respective sequences of HMGB2 3’UTR, CMPK1 3’UTR and lncRNA-OIP5-AS1 fragments containing putative miR-218-5p binding sites (S1: 365–1167;S2: 3078–3539;S3: 4293–4701 and S4: 7775–8169) were amplified by PCR and inserted into the pGL3-Control plasmid (Promega, Madison, WI, USA), respectively. The pCMV6-Entry-C-Myc-p53 construct and pCMV6-Entry-C-Myc construct were provided by ORIGENE (Beijing, China)
FigureFigureFigure
Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma
Ueno, Hirata, Majid et al
Mol. Carcinog. (2011) 50 (6), 449-57 DOI: 10.1002/mc.20729
Cited by 61
AI Summary: OriGene’s transfection-ready DNA for the human jun oncogene (JUN, NM_002228.3) was used in this study to investigate the molecular mechanisms by which Dkk-3 induces apoptosis in renal cell carcinoma cells.
Snippet: well tissue culture plate and transfected with 250 ng of pCMV6-Entry Vector or Homo sapiens jun oncogene (JUN) as transfection-ready DNA NM_002228.3 (ORIGENE), 237.5 ng ... 13 Tsuji T, Miyazaki M, Sakaguchi M, Inoue Y, Namba M. A REIC gene shows down-regulation in
Showing 1-4 of 4 papers.
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