Cas9 NC-NLS Streptococcus Pyogenes Recombinant Protein
SKU
TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
$150.00
In Stock*
Specifications
| Product Data | |
| Species | Streptococcus Pyogenes |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | N-His |
| Predicted MW | 165.0 kDa |
| Concentration | 10 uM |
| Purity | > 90% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol |
| Bioactivity | In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease (Cat# TP790148). The input DNA is XmnI-linearized vector (PS100074) was cleaved by with a synthetic sgRNA. |
| Endotoxin | < 0.5 EU per 1 µg of the protein by the LAL |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -20°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Intronic regulation of SARS-CoV-2 receptor (ACE2) expression mediated by immune signaling and oxidative stress pathways
Richard, Muthuirulan, Aguiar et al
iScience (2022) 25 (7), 104614 DOI: 10.1016/j.isci.2022.104614
Richard, Muthuirulan, Aguiar et al
iScience (2022) 25 (7), 104614 DOI: 10.1016/j.isci.2022.104614
Cited by 14
AI Summary: OriGene’s Human recombinant interferon alpha 2 (TP723881) was used in this study to investigate how immune signaling pathways regulate ACE2 expression, contributing to the functional validation of regulatory elements identified in ACE2 gene regulation.
Snippet: 2 Clinical isolate SARS-CoV-2/SB3 Biological samples ------------------------- Chemicals, peptides, and recombinant proteins ------------------------- Human recombinant interferon alpha 2 OriGene Technologies TP723881 Recombinant human IFNβ1 ThermoFisher Scientific R69007 ------------------------- Critical commercial assays ------------------------- Direct-zol RNA Miniprep
Encapsulating Cas9 into extracellular vesicles by protein myristoylation
Whitley, Kim, Lou et al
J Extracell Vesicles (2022) 11 (4), e12196 DOI: 10.1002/jev2.12196
Whitley, Kim, Lou et al
J Extracell Vesicles (2022) 11 (4), e12196 DOI: 10.1002/jev2.12196
Cited by 62
AI Summary: OriGene’s CRISPR vectors were used as PCR templates to construct plasmids expressing N-myristoylated Cas9, enabling the study’s development of Cas9 encapsulation into extracellular vesicles for genome editing.
Snippet: in EV‐based genome editing therapy. 2 MATERIALS AND METHODS 2.1 Plasmid constructs To create non‐lentiviral vectors expressing N‐myristoylated Cas9 (mCas9), Cas9/sgRNA‐empty, CRISPR vectors (OriGene, Rockville, MD, USA) were used as PCR templates. The Src(WT;8 a.a) (forward primer) and mCas9 primer (reverse primer) (Table S1) were used to obtain a PCR product, which fused the DNA sequenc
Cas9-specific immune responses compromise local and systemic AAV CRISPR therapy in multiple dystrophic canine models
Hakim, Kumar, Pérez-López et al
Nat Commun (2021) 12 (1), 6769 DOI: 10.1038/s41467-021-26830-7
Hakim, Kumar, Pérez-López et al
Nat Commun (2021) 12 (1), 6769 DOI: 10.1038/s41467-021-26830-7
Cited by 180
AI Summary: OriGene’s SpCas9 protein was used as the coating antigen in ELISA assays to measure serum anti-SpCas9 antibody levels, enabling assessment of immune responses to Cas9 in treated dogs.
Snippet: ed in the reaction. Anti-SpCas9 antibody assay Serum anti-SpCas9 antibody levels were evaluated blindly using an enzyme-linked immunosorbent assay (ELISA). Briefly, 25 ng/ml of the SpCas9 protein (OriGene Technologies Inc., Rockville, MD, USA) in carbonate buffer was coated on a microtiter plate. The serum was used at 1:50 dilution. Horseradish peroxidase (HRP) conjugated goat anti-dog IgG was;Br...
Pulsed-electromagnetic-field induced osteoblast differentiation requires activation of genes downstream of adenosine receptors A2A and A3
Kar, Ferguson, Zhang et al
PLoS One (2021) 16 (2), e0247659 DOI: 10.1371/journal.pone.0247659
Kar, Ferguson, Zhang et al
PLoS One (2021) 16 (2), e0247659 DOI: 10.1371/journal.pone.0247659
Cited by 12
AI Summary: OriGene’s CRISPR-Cas9 gene disruption kits were used to knock out the Adora2A and Adora3 genes, enabling the study of their roles in pulsed-electromagnetic-field-induced osteoblast differentiation.
Snippet: Materials and methods. Plasmids and stable lines. Homology Directed Repair (HDR) mediated CRISPR-Cas9 gene disruption technique (Origene Technologies, Inc.) was used to gene knock-out Adora A 2A and A 3 , according to the manufacturer's protocol Cached;- Materials and methods. Plasmids and stable lines. Homology Directed Repair (HDR) mediated CRISPR-Cas9 gene disruption technique (Origen...
Histone deacetylase inhibitors induce medulloblastoma cell death independent of HDACs recruited in REST repression complexes
Alshawli, Wurdak, Wood et al
Mol Genet Genomic Med (2020)
Alshawli, Wurdak, Wood et al
Mol Genet Genomic Med (2020)
Cited by 6
Ultrasound-activated particles as CRISPR/Cas9 delivery system for androgenic alopecia therapy
Ryu, Won, Lee et al
Biomaterials (2020) 232, 119736 DOI: 10.1016/j.biomaterials.2019.119736
Ryu, Won, Lee et al
Biomaterials (2020) 232, 119736 DOI: 10.1016/j.biomaterials.2019.119736
Cited by 115
AI Summary: In this study, OriGene’s SRD5A2 antibody (Cat# TA501321) was used to evaluate the suppression of SRD5A2 protein expression after CRISPR/Cas9-mediated gene editing, thereby helping to assess the therapeutic efficacy of the ultrasound-activated delivery system for androgenic alopecia.
Showing 1-6 of 16 papers.
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