CAS9 Streptococcus Pyogenes Recombinant Protein

CAT#: TP790148

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli


 Product Datasheet for 'TP790148'

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USD 125.00


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    • 500 pmol


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Product Data
Description Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
Species Streptococcus Pyogenes
Expression Host E. coli
Tag N-His
Predicted MW 165.0 kDa
Concentration 20 uM
Purity > 95% as determined by SDS-PAGE and Coomassie blue staining
Buffer 10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol.
Bioactivity In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease (Cat# TP790148). The input DNA is XmnI-linearized vector (PS100074) was cleaved by with a synthetic sgRNA.
Endotoxin < 0.5 EU per 1 µg of the protein by the LAL
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