Lentiviral
Control Particles
Ensure Every Transduction Experiment Is Publication-Ready
Validated positive, negative, and reporter lentiviral control particles to optimize MOI, verify transduction efficiency, and strengthen your experimental data.
OriGene Lentiviral Control Particles - Organized by Application
| Control Type | Description | Promoter | Tag/Reporter | Selection Marker | SKU |
|---|---|---|---|---|---|
| Scrambled shRNA Control | Negative scrambled control for all shRNA Lentiviral particles (cat. no. TLxxxxxxV) | CMV | GFP | Puromycin |
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| CMV | tRFP | Puromycin |
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| CMV | mCherry | Puromycin |
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| Lenti-ORF Control | Negative control for L1V lentiviral particles (cat. no.xxxxxxxxL1V) | CMV | Myc‑DDK | None |
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| Negative control for L2V lentiviral particles (cat. no.xxxxxxxxL2V) | CMV | mGFP | None |
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| Negative control for L3V lentiviral particles (cat. no.xxxxxxxxL3V) | CMV | Myc‑DDK | Puromycin |
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| Negative control for L4V lentiviral particles (cat. no.xxxxxxxxL4V) | CMV | mGFP | Puromycin |
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| Negative control with C‑terminal mRFP and Puro | CMV | mRFP | Puromycin |
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| Negative control with Puro | CMV | None | Puromycin |
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| EF1a promoter negative control with C-terminal mGFP | EF1a | mGFP | None |
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| EF1a promoter negative control with C-terminal mGFP and Puro | EF1a | mGFP | Puromycin |
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| Inducible Expression Control | Tet-On lentiviral control for regulated gene expression studies | Tet-On | GFP | None |
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| Luciferase Reporter Controls | Firefly luciferase lentiviral constructs for reporter assays | CMV | Luciferase | Puromycin |
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Why Use Lentiviral Control Particles?
Validate Transduction Efficiency
Optimize MOI Accurately
Strengthen Experimental Rigor
Troubleshoot with Confidence
Types of Control Particles
Choose the right control for every stage of your lentiviral experiment
Positive Controls
Express well-characterized genes (e.g., GAPDH, Beta-Actin) to confirm your transduction system is functional and delivering transgenes efficiently.
Ideal for: System validation & transduction efficiency confirmation
Negative Controls
Empty vector or non-targeting constructs that confirm observed effects are due to your gene of interest—not the lentiviral backbone itself.
Ideal for: Baseline establishment & off-target effect ruling
Reporter Controls
GFP, RFP, and luciferase reporter particles for real-time visualization of transduction efficiency and MOI optimization by flow cytometry or microscopy.
Ideal for: MOI optimization & transduction monitoring
Applications
From initial setup to publication, control particles support every stage of your research.
Transduction Optimization
MOI Determination
Antibiotic Selection Validation
Assay Controls
Workflow Troubleshooting
From Constitutive to Inducible—Performance Verified
Demonstrated high expression, tight regulation, and robust transduction across a full range of lentiviral control systems.
HEK293 cells (96-well plate) were transduced by different volumes of TR30021V. Fluorescence pictures taken 72 hrs after.
HEK293 cells (96-well plate) were transduced by different volume
of TR30033V. Fluorescence pictures taken 72 hrs after.
HEK293 cells were transduced by Tet-on tetracycline-inducible GFP expression lentiviral particles ().
Without Dox: Expression of GFP is not observed when Dox is absent from the culture medium.
With Dox: Expression of GFP is observed when Dox is added into medium.
Control Particle Workflow
A simple 5-step process to validate your lentiviral system before running experiments.
STEP 01
Seed Cells
Plate target cells at optimal density for tranduction.
STEP 02
Transduce
Add control lentiviral particles at desired MOI.
STEP 03
Monitor Reporter
Observe GFP/RFP expression or apply selection
STEP 04
Optimize MOI
Quantify efficiency and determine optimal conditions.
STEP 05
Validate
Proceed with experimental vector using optimized protocol.
Technical Specifications
Detailed specifications for lentiviral control particles.
Titer Information
Standard titer: ≥107 TU/mL (ready-to-use) or ≥108 TU/mL (concentrated). Functional titer verified by flow cytometry or antibiotic selection in HEK293T cells.
Quality Control & Validation
Every lot undergoes qPCR-based titer quantification, sterility testing, mycoplasma testing (negative), and functional validation by transduction of target cells. Certificate of Analysis included.
Storage & Handling
Store at −80°C upon receipt. Avoid repeated freeze-thaw cycles. Aliquot upon first thaw for long-term storage. Stable for ≥6 months at −80°C.
Biosafety Level
BSL‑2. OriGene lentiviral particles are replication‑incompetent (3rd generation system with self‑inactivating LTR). Follow institutional biosafety guidelines.
Packaging & Formats
Available as ready‑to‑use (200 µL) or concentrated (50 µL) formats. Custom packaging sizes available upon request for high‑throughput applications.
Why Researchers Trust OriGene
Industry-leading quality, reproducibility, and technical support backed by over two decades of molecular biology expertise.
QC Validated
Every lot is functionally tested
30+ Years
Experience in gene research tools
Peer-Reviewed
Cited in thousands of publications
