OriGene Logo

EIF3S3 (EIF3H) (NM_003756) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC201767
EIF3H (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit H (EIF3H)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA349924 Rabbit Polyclonal Anti-EIF3H Antibody
100 ul
$205.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags 30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "EIF3S3 or NM_003756"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF3S3
Synonyms eIF3-gamma; eIF3-p40; EIF3S3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201767 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGCGCAGCAGGGA
AAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAGATAGATGGCCTTGTGGTATT
AAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACTGAAGTTGTTCAAGGAGTGCTTTTGGGTCTG
GTTGTAGAAGATCGGCTTGAAATTACCAACTGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACT
TTGATGAAGTCCAATATCAGATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGT
GGGCTGGTATCAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGATCTCTCTCAC
TAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAGGATTTTTCCCCTGAAGCATT
GAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAAGTGCCGATTGTAATTAAAAATTCACATCTG
ATCAATGTCCTAATGTGGGAACTTGAAAAGAAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTG
CCAGCAGCAATCATTTGGGGAAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATAT
AGTTAAATACAACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGACCTGTCCAAAC
TCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCAGGCCAGATAAACACTTACTG
CCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAGCTCTTCATGGCCCAGGCTCTTCAAGAATAC
AACAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201767 protein sequence
Red=Cloning site Green=Tags(s)

MASRKEGTGSTATSSSSTAGAAGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGTEVVQGVLLGL
VVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSY
QHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHL
INVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQR
RQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEY
NN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003756
ORF Size 1056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003756.3
RefSeq Size 1286 bp
RefSeq ORF 1059 bp
Locus ID 8667
UniProt ID O15372
Cytogenetics 8q23.3-q24.11
Domains JAB_MPN
MW 39.9 kDa
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_003756" in other vectors (6)
SKU Description Size Price
RC201767L1 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), Myc-DDK-tagged 10 ug
$757.00
RC201767L2 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), mGFP tagged 10 ug
$757.00
RC201767L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), Myc-DDK-tagged 10 ug
$757.00
RC201767L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), mGFP tagged 10 ug
$757.00
RG201767 EIF3H (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit H (EIF3H) 10 ug
$489.00 $657.00
SC127130 EIF3H (untagged)-Human eukaryotic translation initiation factor 3, subunit H (EIF3H) 10 ug
$457.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img
 

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.