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EIF3S3 (EIF3H) (NM_003756) Human Untagged Clone

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SKU
SC127130
EIF3H (untagged)-Human eukaryotic translation initiation factor 3, subunit H (EIF3H)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EIF3S3
Synonyms eIF3-gamma; eIF3-p40; EIF3S3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127130 sequence for NM_003756 edited (data generated by NextGen Sequencing)
ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAACTAA

Clone variation with respect to NM_003756.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003756 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGAAAGAGGCGT
CCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGCGCAGCAG
GGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAGATAGATG
GCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACTGAAGTTG
TTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAACTGCTTTC
CTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAGATGGAAA
TGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTATCAGTCCA
CATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTACCAGCATG
CCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGATCTCTCT
CACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAGGATTTTT
TCCCCTTGAAGCATTGAAAAAAGCANATATCACCTTTGAGTACATGTTTGAAGAAGTGCC
GATTGTAATTAANAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAGAAGTC
AGCTGTTGCAGATAACATGAATTGCTCAGCCTTGCCAGCAGNCATCATTTGGGGAAGAAT
CTACANGTGCTGATGGACAGAGTGGATGAAATGAGCCAGNAATAGNTAANTACACCACTA
CATGAGGAATACTAGTAAACACGCAGCAGAAACTCAGTTCAGCAGCGGTCGCAGCAGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003756 unedited
TTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAAGATTTGTTT
TATTTTATTATGGCTAGAAAAACACTGTTATAGCCAAAATCGGCAATGACACTAAAGAAA
TCCTCTGTGCTTTTCAATATGCAAATATATTTCTTCCAAGAGTTGCCCTGGTGTGACTTC
AAGAGTTCATGTTAACTTCTTTTCTGGAAACTTCCTTTTCTTAGTTGTTGTATTCTTGAA
GAGCCTGGGCCATGAAGAGCTTGCCTAAGTTTTGGGCAGTGAACTCCTTGATGTTCTGGC
AGTAAGTGTTTATCTGGCCTGCAATGAGCAGCGAGTCCATCCTGGCAGGCGGCTGTGGTG
GTTTGAAAAGTTTGGACAGGTCCTCCTCAGGGAGCGGGGGTTCTCCTCGGCTCTGGCGCT
GCATATTCTCCTGCTGGCGACGCTGCTGATACTGATGTTTCTGCTGCTGTTGTTTACTAG
TATTCCTCATGTATGTGTTGTATTTAACTATATCTTGGCTCATTTCATCCACTCTGTCCA
TCAGCAACTGTAGATTCTTCCCCAAATGATTGCTGCTGGCAAGGCTGAGCAATTCATGTT
TATCTGCAACAGCTGACTTCTTTTCAAGTTCCCACATTAGGACATTGATCAGATGTGAAT
TTTTAATTACAATCGGCACTTCTTCAAACATGTACTCAAAGGTGATATTTGCTTTTTTCA
ATGCTTCAGGGGAAAAATCCTTTTCTTTACAAACTTCCATCAGTTTAGGAGTCAGTCTGT
ATGCCTTTAGTGAGAGAGATCCTTGGGCAGTTTTNATGGGATCATAAATGANAACGACAG
ATTCTTCATGGGCATGCTGTAACTAAACTGAAAGTCCANGAGTGCCCCGGGTACGAATGA
CCCATANTTTGGGGACTGATACCANCCCAGTGAAAAAGATCATGTTACATGGCGAAAGCT
CCGCATAATTCCATCTGAATGGACTCA
Restriction Sites NotI-NotI |
ACCN NM_003756
Insert Size 1300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003756.1, NP_003747.1
RefSeq Size 1280 bp
RefSeq ORF 1059 bp
Locus ID 8667
UniProt ID O15372
Cytogenetics 8q23.3-q24.11
Domains JAB_MPN
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
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"NM_003756" in other vectors (6)
SKU Description Size Price
RC201767 EIF3H (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit H (EIF3H) 10 ug
$457.00
RC201767L1 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), Myc-DDK-tagged 10 ug
$757.00
RC201767L2 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), mGFP tagged 10 ug
$757.00
RC201767L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), Myc-DDK-tagged 10 ug
$757.00
RC201767L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit H (EIF3H), mGFP tagged 10 ug
$757.00
RG201767 EIF3H (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit H (EIF3H) 10 ug
$489.00 $657.00

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