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NIPSNAP3B (NM_018376) Human Tagged ORF Clone

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SKU
RC205012
NIPSNAP3B (Myc-DDK-tagged)-Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "NIPSNAP3B or NM_018376"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NIPSNAP3B
Synonyms FP944; NIPSNAP3; SNAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205012 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCGTTCTCAGAAGCGGCCTGACCAAGGCGCTTGCCTCACGGACGCTCGCGCCTCAGGTGTGTTCAT
CTTTTGCTACGGGCCCTAGACAATACGATGGAACGTTCTATGAATTTCGTACTTATTACCTTAAACCTTC
AAATATGAATGCGTTCATGGAAAATCTTAAGAAAAACATTCATCTTCGGACCTCTTACTCTGAATTGGTT
GGATTCTGGAGTGTAGAATTTGGAGGCAGAACGAATAAAGTGTTTCATATTTGGAAGTATGATAATTTTG
CTCATCGAGCTGAAGTTCGGAAAGCCTTAGCCAACTGTAAGGAATGGCAAGAACAATCTATCATTCCAAA
TTTGGCTCGCATTGATAAACAAGAGACGGAAATTACTTACCTGATACCATGGTCCAAATTAGAAAAGCCT
CCAAAAGAAGGAGTCTATGAACTAGCTGTTTTTCAGATGAAACCTGGTGGGCCAGCTCTGTGGGGTGATG
CATTTGAAAGAGCAATTAATGCCCATGTCAATTTAGGCTACACAAAAGTAGTTGGTGTTTTCCACACAGA
ATATGGAGAACTCAACAGAGTTCATGTTCTTTGGTGGAATGAGAGTGCAGATAGTCGTGCAGCTGGGAGA
CATAAGTCCCATGAGGATCCCAGAGTTGTGGCGGCTGTTCGGGAAAGTGTCAACTACCTAGTTTCTCAGC
AGAATATGCTTCTGATTCCTGCATCATTTTCACCATTGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205012 protein sequence
Red=Cloning site Green=Tags(s)

MLVLRSGLTKALASRTLAPQVCSSFATGPRQYDGTFYEFRTYYLKPSNMNAFMENLKKNIHLRTSYSELV
GFWSVEFGGRTNKVFHIWKYDNFAHRAEVRKALANCKEWQEQSIIPNLARIDKQETEITYLIPWSKLEKP
PKEGVYELAVFQMKPGGPALWGDAFERAINAHVNLGYTKVVGVFHTEYGELNRVHVLWWNESADSRAAGR
HKSHEDPRVVAAVRESVNYLVSQQNMLLIPASFSPLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018376
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018376.4
RefSeq Size 1781 bp
RefSeq ORF 744 bp
Locus ID 55335
UniProt ID Q9BS92, Q71RE8
Cytogenetics 9q31.1
MW 28.3 kDa
Summary NIPSNAP3B belongs to a family of proteins with putative roles in vesicular trafficking (Buechler et al., 2004 PubMed 15177564).supplied by OMIM, Mar 2008
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Resources
"NM_018376" in other vectors (5)
SKU Description Size Price
RC205012L3 Lenti ORF clone of Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B), Myc-DDK-tagged 10 ug
$600.00
RC205012L4 Lenti ORF clone of Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B), mGFP tagged 10 ug
$600.00
RG205012 NIPSNAP3B (tGFP-tagged) - Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B) 10 ug
$489.00 $500.00
SC113524 NIPSNAP3B (untagged)-Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B) 10 ug
$300.00
SC320801 NIPSNAP3B (untagged)-Human nipsnap homolog 3B (C. elegans) (NIPSNAP3B) 10 ug
$300.00

Plasmid Map for RC205012

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205012
Circular map for RC205012

file-alt Citations

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