Phospho-STAT

Western blot analysis of lysates from HeLa cells

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (TA420055) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.

Western blot using affinity purified anti-Stat2pY690 antibody shows detection of Stat2pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000U/mL of IFN-a for 15 min at 37oC. Primary antibody was used at 1:1,000.

Western blot analysis of lysates from NIH/3T3 cells

Immunofluorescent analysis of A431 cells treated with EGF, using Phospho-STAT3 (S727) Antibody .

Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.

Western blot analysis of Phospho-STAT3 (Ser727) in various lysates using Phospho-STAT3 (Ser727) antibody.

Western blot analysis of Phospho-STAT3 (Tyr705) in various lysates using Phospho-STAT3 (Tyr705) antibody.

Western blot analysis of Phospho-STAT1 (Tyr701) in various lysates using Phospho-STAT1 (Tyr701) antibody.

Western blot analysis of lysates from HeLa cells

Immunohistochemistry using Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.

Western blot analysis of Phospho-STAT1 (Ser727) in Hela, Hela+UV, C6, C6+UV lysates using Phospho-STAT1 (Ser727) antibody.

Western blot analysis of lysates from HeLa cells

Western blot (WB) analysis of p-Stat5 (S726/731) pAb at 1:500 dilution Lane1:L02 whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)

Western blot analysis of Phospho-STAT3 (Ser727) in Jurkat, C6, Hela lysates using Phospho-STAT3 (Ser727) antibody.

Western blot analysis of Phospho-Stat5 (Y694) expression in A431 cell lysate treated with EGF.

Western blot (WB) analysis of p-Stat3 (Y705) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:The Embryo tissue lysate of Mouse(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Western blot analysis of Phospho-STAT1 (Y701) expression in (1) A431 cell lysate; (2) A431 lysate cell treated with EGF.

Western blot (WB) analysis of p-Stat3 (S727) pAb at 1:2000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)