Research Areas

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DDX58 (Cat# RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58(Cat# TA506141). Positive lysates LY415370 (100ug) and LC415370 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Circular map for RC222515

SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGLL (RC218358, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGLL. Positive lysates LY402124 (100ug) and LC402124 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGM2 (Cat# RC217353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGM2 (Cat# TA809245). Positive lysates LY401458 (100ug) and LC401458 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-cJUN clone UMAB67 mouse monoclonal antibody. HIER ACCEL buffer (B22C-125) (pH8.7) at 110C for 10 min, UM800011 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2A6 (Cat# RC222995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6(Cat# TA503857). Positive lysates LY424530 (100ug) and LC424530 (20ug) can be purchased separately from OriGene.

Flow cytometric analysis of living 293T cells transfected with BTLA overexpression plasmid (RC219458), Red)/empty vector (PS100001, Blue) using anti-BTLA antibody (TA812577). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

RC201983L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC201983L4V particle to overexpress human RAF1-mGFP fusion protein.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1. Positive lysates LY409966 (100ug) and LC409966 (20ug) can be purchased separately from OriGene.

Circular map for RC211823

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTRK1 (Cat# RC213047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK1(Cat# TA806413). Positive lysates LY419273 (100ug) and LC419273 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (RC223612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP. Positive lysates LY401576 (100ug) and LC401576 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RCAN3 (Cat# RC207755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RCAN3(Cat# TA505496). Positive lysates LY415541 (100ug) and LC415541 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RFESD (Cat# RC207317, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFESD(Cat# TA505809). Positive lysates LY403551 (100ug) and LC403551 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. Positive lysates LY418732 (100ug) and LC418732 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP3 (RC209597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP3 (1:2000). Positive lysates LY416563 (100ug) and LC416563 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYNN (RC207080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYNN. Positive lysates LY412964 (100ug) and LC412964 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLTA (RC203505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLTA (1:2000). Positive lysates LY400696 (100ug) and LC400696 (20ug) can be purchased separately from OriGene.

Circular map for MR219259

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1. Positive lysates LY410744 (100ug) and LC410744 (20ug) can be purchased separately from OriGene.

Circular map for MR203848

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1. Positive lysates LY403455 (100ug) and LC403455 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM4 (RC213480, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM4 (1:2000). Positive lysates LY422267 (100ug) and LC422267 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (RC204369, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polycl. Positive lysates LY408504 (100ug) and LC408504 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2. Positive lysates LY417979 (100ug) and LC417979 (20ug) can be purchased separately from OriGene.

Figure 2. Western blot analysis od Dityrosine -cross-linked Human Amyloid Beta. 200 ng of Abeta 1-40 peptides before and after Cu (II)-Oxidation were separated by SDS-PAGE and immunblotted with AM20243PU-S. AM20243PU-S detects dimeric and trimeric Abeta 1-4

Circular map for MG226319

Circular map for MR227486

Circular map for RC214278L1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8 (1:500). Positive lysates LY401593 (100ug) and LC401593 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.

DNA - 4x.

DNA - 4x.

Circular map for RC212501L1

Circular map for RC216727L1

Circular map for RG216428

Circular map for RG222681

IL17REL Human Gene Knockout Kit (CRISPR)

Ccl26 Mouse Gene Knockout Kit (CRISPR)

Siva1 Mouse Gene Knockout Kit (CRISPR)