Phospho-p53

Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit pAb (A3185). HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.

Western blot analysis of Phospho-p53 (Ser15) in HeLa treated with HU lysates using Phospho-p53 (Ser15) antibody.The lane on the right is blocked with the Phospho- peptide.

Western blot analysis of lysates from 293T cells using Phospho-p53-S15 Rabbit pAb (TA382720) at 1:900 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

Western blot analysis of lysates from 293 and HT-29 cells

Western blot analysis of various lysates using Phospho-p53-S37 Rabbit pAb (TA382728) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

Western blot analysis of Phospho-p53 (Ser6) in A549, MCF-7 lysates using Phospho-p53 (Ser6) antibody.

Western blot analysis of lysates from NIH/3T3 cells

Western blot analysis of Phospho-p53 (Ser392) in HEK293, A431 lysates using Phospho-p53 (Ser392) antibody.

Western blot analysis of lysates from MCF7 cells

Western blot analysis of lysates from HeLa cells

Western blot analysis of lysates from C6 cells

Western blot analysis of Phospho-p53 (Ser33) in T47D cell lysates treated with etoposide.

Western blot (WB) analysis of p53 (Phospho-S46) polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug）

Western blot analysis of Phospho-p53 (Ser9) expression in (1)HepG2 cell lysates treated with etoposide; (2)HeLa cell lysates treated with etoposide.

Western blot analysis of Phospho-p53 (T55) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid.

Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid.