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Phospho-SMAD

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  • Western blot analysis of lysates from HeLa cells
    3 Weeks*
    $475.00
    TA381748 - Phospho Smad3 (Thr179) Rabbit Polyclonal Antibody
    Phospho-Smad3-T179 Rabbit polyclonal Antibody
    Applications ELISA, IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
  • Western blot analysis of lysates from C6 cells
    3 Weeks*
    $475.00
    TA420056 - Phospho-SMAD3 Rabbit Monoclonal Antibody
    Phospho-Smad3-S423/S425 Rabbit mAb
    Applications ELISA, IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
  • Western blot analysis of Phospho-Smad3 (Ser425) in various lysates using Phospho-Smad3 (Ser425) antibody.
    4 Weeks*
    $380.00
    TA385271 - Phospho Smad3 (Ser425) Rabbit Polyclonal Antibody
    Phospho-Smad3 (Ser425) Rabbit polyclonal Antibody
    Applications ELISA, ICC, IF, IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425 (Phosphorylated)
  • Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
    2 Weeks*
    $870.00
    TA319191 - Phospho SMAD3 (Ser423/425) Rabbit Polyclonal Antibody
    Rabbit polyclonal SMAD3 phospho S423/phospho S425 antibody
    Applications IHC, WB
    Reactivities Bovine, Chicken, Human, Mouse, Pig, Rat, X. tropicalis, Xenopus, Zebrafish
    Conjugation Unconjugated
    Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
  • Western blot analysis of Phospho-Smad2 (Ser255) in K562 lysates using Phospho-Smad2 (Ser255) antibody.
    4 Weeks*
    $380.00
    TA385265 - Phospho Smad2 (Ser255) Rabbit Monoclonal Antibody [Clone ID: R02-8F2]
    Phospho-Smad2 (Ser255) Rabbit monoclonal Antibody
    Applications IHC, IP, WB
    Reactivities Human
    Conjugation Unconjugated
    Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser255 of human Smad2 (Phosphorylated)
  • Western blot analysis of various lysates using (TA381749) at 1:1000 dilution. Hela cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
    3 Weeks*
    $475.00
    TA381749 - Phospho Smad3 (Ser423/425) Rabbit Polyclonal Antibody
    Phospho-Smad3-S423/S425 Rabbit polyclonal Antibody
    Applications ELISA, IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
  • Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using Phospho-Smad2 (S255) Antibody.
    4 Weeks*
    $480.00
    TA422758 - Phospho Smad2 (Ser255) Rabbit Monoclonal Antibody [Clone ID: OTIRA0577]
    Phospho-Smad2 (S255) Antibody
    Applications IHC, IP, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthesized peptide derived from human Smad2
  • Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody.
    4 Weeks*
    $480.00
    TA422260 - Phospho Smad5 (Ser463/465) Rabbit Monoclonal Antibody [Clone ID: OTIRA0079]
    Phospho-Smad5 (S463/465) Antibody
    Applications ICC/IF, IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthesized peptide derived from human Phospho-Smad5 (S463/465)
  • Western blot analysis of various lysates
    3 Weeks*
    $475.00
    TA420041 - Phospho-SMAD2 Rabbit Monoclonal Antibody
    Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb
    Applications ELISA, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
  • Western blot analysis of lysates from NIH/3T3 cells
    3 Weeks*
    $475.00
    TA420109 - Phospho-SMAD5 Rabbit Monoclonal Antibody
    Phospho-Smad5-S465 Rabbit mAb
    Applications ELISA, WB
    Reactivities Mouse, Rat
    Conjugation Unconjugated
  • Western blot analysis of Phospho-Smad2/3 (Thr8) in various lysates using Phospho-Smad2/3 (Thr8) antibody.
    4 Weeks*
    $380.00
    TA385267 - Phospho Smad2/3 (Thr8) Rabbit Polyclonal Antibody
    Phospho-Smad2/3 (Thr8) Rabbit polyclonal Antibody
    Applications ELISA, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. AA range:1-50 (Phosphorylated)
  • Western blot (WB) analysis of p-Smad1 (S465) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug) Lane3:A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug) Lane4:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane5:A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:The Uterus tissue lysate of Mouse(40ug) Lane7:The Uterus tissue lysate of Rat(40ug)
    7 Weeks*
    $340.00
    TA392948 - Phospho Smad1 (Ser465) Rabbit Polyclonal Antibody
    Smad1 (phospho-S465) polyclonal antibody
    Applications IHC, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465.
  • Western blot analysis of Phospho-Smad2 (Ser250) in mouse brain, mouse lung lysates using Phospho-Smad2 (Ser250) antibody.
    4 Weeks*
    $320.00
    TA421966 - Phospho Smad2 (Ser250) Rabbit Monoclonal Antibody [Clone ID: R02-0D1]
    Phospho-Smad2 (Ser250) Rabbit mAb
    Applications WB
    Reactivities Hamster, Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser250 of human Smad2 (Phosphorylated)
  • Immunohistochemistry analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (Ser425) (7H6) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
    4 Weeks*
    $380.00
    TA385269 - Phospho Smad3 (Ser425) (7H6) Mouse Monoclonal Antibody [Clone ID: 7H6-4E5-4H2]
    Phospho-Smad3 (Ser425) (7H6) Mouse monoclonal Antibody
    Applications IHC
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen Synthetic peptide conjugated to KLH. (Phosphorylated)
  • Western blot analysis of various lysates
    3 Weeks*
    $475.00
    TA420071 - Phospho-SMAD2 Rabbit Monoclonal Antibody
    Phospho-Smad2-S245/S250/S255 Rabbit mAb
    Applications ELISA, WB
    Reactivities Human
    Conjugation Unconjugated
  • Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate.
    4 Weeks*
    $480.00
    TA422752 - Phospho Smad5 (Ser463/465) Rabbit Monoclonal Antibody [Clone ID: OTIRA0571]
    Phospho-Smad5 (S463/S465) Antibody
    Applications ICC/IF, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthesized peptide derived from human Smad5
  • Western blot analysis of lysates from HeLa cells
    3 Weeks*
    $475.00
    TA381741 - Phospho Smad2 (Ser465/467) Rabbit Polyclonal Antibody
    Phospho-Smad2-S465/S467 Rabbit polyclonal Antibody
    Applications ELISA, WB
    Reactivities Human, Mouse
    Conjugation Unconjugated
  • Western Blot of Rabbit anti-Smad2 pS465pS467 antibody. Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-β1 for 1h. Load: 20 µg per lane. Primary antibody: Smad2pS465pS467 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 52.3 kDa for Smad2pS465pS467. Other band(s): ~85kDa non-specific band.
    5 Days*
    $300.00
    TA397537S - Phopho SMAD2 (Ser465/467) Rabbit Polyclonal Antibody
    SMAD2 phospho S465/phospho S467 Antibody
    Applications ELISA, WB
    Reactivities Human
    Conjugation Unconjugated
    Immunogen SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
  • Western blot analysis of various lysates
    3 Weeks*
    $475.00
    TA420073 - Phospho-SMAD2 Rabbit Monoclonal Antibody
    Phospho-Smad2-S465/S467 Rabbit mAb
    Applications ELISA, WB
    Reactivities Human, Mouse
    Conjugation Unconjugated
  • Western blot analysis of Phospho-Smad1 (Ser463/Ser465) in A549, U2OS, C6 lysates using Phospho-Smad1 (Ser463/Ser465) antibody.
    4 Weeks*
    $320.00
    TA421967 - Phospho Smad1 (Ser463/Ser465) Rabbit Monoclonal Antibody [Clone ID: R07-4A3]
    Phospho-Smad1 (Ser463/Ser465) Rabbit mAb
    Applications IP, WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of human Smad1 (Phosphorylated)
  • Western blot (WB) analysis of p-Smad2/3 (T8) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40µg) Lane2:Hela whole cell lysate(40µg) Lane3:The Lung tissue lysate of Mouse(40µg) Lane4:SGC7901 whole cell lysate(40µg) Lane5:H9C2 whole cell lysate(40µg)
    7 Weeks*
    $340.00
    TA392951 - Phospho Smad2/3 (Thr8) Rabbit Polyclonal Antibody
    Smad2/3 (phospho-T8) polyclonal antibody
    Applications WB
    Reactivities Human, Mouse
    Conjugation Unconjugated
    Immunogen Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
  • Western blot (WB) analysis of p-Smad3 (S204) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
    7 Weeks*
    $340.00
    TA392657 - Phospho Smad3 (Ser204) Rabbit Polyclonal Antibody
    Smad3 (phospho-S204) polyclonal antibody
    Applications WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 204.
  • NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 (RABBIT) Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.
    5 Days*
    $870.00
    TA319189 - Phospho SMAD3 (Thr179) Rabbit Polyclonal Antibody
    Rabbit polyclonal SMAD3 phospho T179 antibody
    Applications WB
    Reactivities Human
    Conjugation Unconjugated
    Immunogen This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
  • Western blot analysis of Phospho-Smad3 (Ser423/425) in Jurkat, C6, CHO-K1, Hela lysates using Phospho-Smad3 (Ser423/425) antibody.
    4 Weeks*
    $380.00
    TA385273 - Phospho Smad3 (Ser423/425) Rabbit Monoclonal Antibody [Clone ID: R09-6A2]
    Phospho-Smad3 (Ser423/425) Rabbit monoclonal Antibody
    Applications WB
    Reactivities Hamster, Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser423/Ser425 of human Smad3 (Phosphorylated)
  • Western blot (WB) analysis of Smad2/3 (phospho-T8) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 10 min whole cell lysate Lane3:The Heart tissue lysate of Mouse
    7 Weeks*
    $340.00
    TA392656 - Phospho Smad2/3 (Thr8) Rabbit Polyclonal Antibody
    Smad2/3 (phospho-T8) polyclonal antibody
    Applications WB
    Reactivities Human, Mouse, Rat
    Conjugation Unconjugated
    Immunogen Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Items 1-25 of 27