Phospho-ERK

Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (TA420033) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. 293T cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Western blot analysis of Phospho-PERK (Thr982) in A453, AD293, Hela lysates using Phospho-PERK (Thr982) antibody.

LO2 cell, treated with LPS(1000ng/ml) for 15 miniutes, untreated or λ phosphatase-treated.The western blot was probed using p-ERK1/2 (Y204) pAb #TA392537 at 1:500 dilution.

Western blot (WB) analysis of ERK1/2 (Phospho-T202/Y204) polyclonal antibody at 1:5000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with PMA(200nM, 10 min) whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 (Tyr222/Tyr205) (1H4) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 (Tyr222/Tyr205) (10C8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-ERK1 (Thr202)/ERK2 (Thr185) in Hela, Hela+TPA, C6, C6+TPA lysates using Phospho-ERK1/2 (Thr202/Thr185) antibody.

Western blot detection of Erk1 (pT202/pY204)/Erk2 (pT185/pY187) in C6,Hela cell lysates using Erk1 (pT202/pY204)/Erk2 (pT185/pY187) Rabbit mAb(1:1000 diluted).Predicted band size:41kDa.Observed band size:44,42kDa.

Western blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and Hydroxyurea-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Rabbit pAb (301245,1:1000 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246-4F3,1:1000 diluted,lower).

Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).

Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes 5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).