Double Staining Protocol Mouse and Rabbit antibodies on human tissue

Double staining is a standard method used in immunohistochemistry to evaluate two distinct antigens in a single tissue.

Key Features

  • OriGene Double Stain Kit is Plug (your primary antibodies & tissue). Everything else is in the kit!
  • Your target antibodies can be used at the same titer as a single stain.
  • There is no conjugation of your primary antibodies
  • Secondary and chromogens are already optimized.
  • Tested on paraffin-embedded human tissue.
  • Multiple Color combination: red & brown; red & purple; red & green
  • Same-day results
Download the protocol here

This protocol gives a step-by-step guide on how to use Double staining kits for IHC. OriGene double staining kit contains

  • HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, biotin free.
  • Two distinct chromogens. DAB chromogen (brown color) is used with the HRP-Polymer anti-Mouse IgG, and GBI-Permanent Red(red color) is used with the AP-Polymer anti-Rabbit IgG.
  • Simplified steps offer a much faster protocol as the enzyme conjugates are applied to the specimen as a mixture.

Tissue Preparation Before Double Stain Protocol:

  1. Fixation: To ensure the quality of the staining and obtain reproducible performance, the user needs to supply appropriately fixed tissue and prepared slides.
  2. FFPE Tissue should be baked or frozen must fixed appropriately for the tissue adhered to the slide tightly to avoid tissue falling off.
  3. FFPE Paraffin-embedded section must be deparaffinized with xylene and rehydrated with a graded series of ethanol before staining.
  4. Cell smear samples should be made as much monolayer as possible to obtain satisfactory results.

Thoughts for Experimental design Before Double Stain Protocol:

  1. Primary antibody test should be done once using the kit. Run each primary alone with both secondary to show primary antibody will not cause background the secondary to other host species.
  2. Three control slides will aid the interpretation of the result: positive tissue control, reagent control (slides treated with Isotype control reagent), and negative control.
  3. Proceed with IHC staining: After deparaffined and especially after HIER do not let the specimen or tissue dry from this point on.
  4. We recommend TBS-T to be used as the wash buffer to get the highest sensitivity and clean background. Phosphate in the PBS-T may inhibit the activity of the alkaline phosphatase.
  5. Note: 1X TBS-T =50mM Tris HCl, 150mM NaCl, 0.05% Tween-20 pH7.6. GBI sells 10xTBS-T for your convenience (B11xx)

* You can use up to 100ul of polymer conjugate per slide.

* Protocol is suitable when both mouse and primary rabbit antibodies need or do not need pre-treatment steps.

Protocol

Reagent Staining Procedure Incubation Time
1. Peroxidase and Alkaline Phosphatase Blocking Reagent Not provided. We recommend using GBI Dual Block E36xx. Fast, easy, and it will block endogenous alkaline phosphatase a. Incubate slides in peroxidase and alkaline phosphatase blocking reagent. We recommend GBI Dual Block E36xx. b. Rinse the slide using distilled water at least twice. 10 min.
2. HIER Pretreatment: Refer to antibody data sheet. a. Heat Induced Epitope Retrieval (HIER) may be required for primary antibody suggested by vendor. b. Wash with PBS-T containing 0.05% Tween-20 or 1X TBS-T(See note 7 above); 3 times for 2 minutes each.
3. Preblock (optional) For the paraffin section, the Improved formula saves the need for a preblock step. For frozen tissue, preblock may or may not be required depending on the fixative. ( Preblock catalog No.:E07 was Recommended. )
4. Mouse antibody 1 and Rabbit antibody 2: Supplied by user Notes: The investigator needs to optimize dilution and incubation times before double staining. a. Apply two drops or enough volume of Primary Antibody 1 and Antibody 2 to cover the tissue completely. Mix well on the slide and Incubate in a moist chamber for 30-60 min. b. Wash with PBS-T containing 0.05% Tween-20 or 1X TBS-T; 3 times for 2 minutes each. 30-60 min.
5. Reagent 1 and 2: Reagent 1 : HRP Polymer anti-Mouse (RTU) Reagent 2: AP Polymer anti-Rabbit (RTU) a. Apply 1 drop (50μl) of Reagent 1 (HRP Polymer anti-Mouse) and one drop of Reagent 2 (AP Polymer anti-Rabbit) to cover each section, and mix well on the slide. Or you may prepare secondary antibodies cocktail in advance: 50μl Reagent 1 (HRP Polymer anti-Mouse) plus 50μl Reagent 2 (AP Polymer anti-Rabbit) per slide. B. Incubate in a moist chamber for 30 min. c. Wash with PBS-T containing 0.05% Tween-20 or 1X TBS-T; 3 times for 2 minutes each. 30 min
6. Reagents 3A, 3B: Reagent 3A: DAB Substrate(RTU) Reagent 3B: DAB Chromogen(20x) a. Add 1 or 2 drops (for higher sensitivity and contrast) of Reagent 3B to 1 mL of Reagent 3A. Mix well. Protect from light and use within 7 hours. B. Apply two drops or enough volume of DAB CHROMOGEN to completely cover the tissue. Incubate for 5 min. c. Rinse thoroughly with distilled water. d. Wash with 1X TBS-T only; 3 times for 2 minutes each. 5 min
7. Reagent 4A, 4B, 4C Reagent 4A: GBI-Permanent Red Substrate (RTU) Reagent 4B: GBI-Permanent Red Activator (5x) Reagent 4C: GBI-Permanent Red Chromogen (100x) (To get maximum sensitivity of AP polymer, Please repeat chromogen step) Note: Make a fresh working solution and use it immediately. Shake GBIPermanent Red Activator well before adding it to GBI-Permanent Red Substrate. A. Add 200μL of Reagent 4B (Activator-shake well) into 1mL of Reagent 4A (Substrate buffer) and mix well. Add 10μL of Reagent 4C (Chromogen) into the mixture and mix well. [Note: For fewer slides, Add 100μL of Reagent 4B (Activator) into 500μL of Reagent 4A (Substrate buffer) and mix well. Add 5μL of Reagent 4C (Chromogen) into the mixture and mix well.] b. Apply two drops (100μL) or enough GBI-Permanent Red working solution to cover the tissue completely. Incubate for 10 min, and observe appropriate color development. To increase the AP signal, make a fresh working solution again, tap off the previous chromogen, apply 2-3 drops (100μL) immediately and incubate an additional 10min. c. Rinse well with distilled water. 10 min OR (10min + 10 min)
8. HEMATOXYLIN Not provided a. Counterstain with two drops (100μl) or enough volume of hematoxylin to cover the tissue completely. Incubate for 10-15 seconds. B. Rinse thoroughly with tap water for 2-3 min. c. Put slides in PBS until they show blue color (about ½ - 1 min.) d. Rinse well in distilled water 10-15 sec
9. Reagent 5: Simpo-Mount(RTU) a. Apply 2 drops (100μL) or enough volume of Reagent 5 Simpo-Mount to cover tissue when tissue is wet. Rotate the slides to allow SimpoMount spread evenly. b. Place slides horizontally in an oven at 40-50°C for at least 30 minutes or leave it at room temperature until slides are thoroughly dried.

Protocol Notes:

1. The fixation, tissue slide thickness, antigen retrieval, primary antibody dilution, and incubation time affect results significantly. The investigator must consider all factors and determine optimal conditions when interpreting the result.

2. GBI-Permanent Red is insoluble in organic solvent and can be coverslipped as well. However, the dehydration steps must be shorter for optimal tissue structure and chromogen signal maintenance. Note: Please wipe off extra water and air dry slides before dehydration and clear. Use fresh ethanol and xylene.

a.1x 80% Ethanol 20 seconds;

b.1x 95% Ethanol 20 seconds;

c.3x 100% Ethanol 20 seconds each;

d.1x 100% Xylene 20 seconds;

e.Add one drop of xylene-based mountant (Cat. No. O-Mount, E02-18) and coverslip. Press to push the air bubble out. CAUTION: DO NOT dehydrate in xylene for longer than 20 seconds! It will erase the GBI-Permanent Red stain!

Work Sheet for your Kit

We designed these worksheets to help you track each step. When staining fails, these sheets help our technical support staff to pinpoint the problem. To ensure that all steps are done correctly, we recommend that the user fill in the actual time of their experimental step and any variation. Results will vary if time recommendations are not followed. RTU translates to ready-to-use

  • Used for the tester to check “√ “each step during the experiment
  • Steps follow after de-paraffinization
  • Refer to insert for details of each step
Download worksheet
Protocol Step Reagent / time Experiment 1 Date: Experiment 2 Date: Experiment 3 Date: Experiment 4 Date:
Step 1 Peroxidase and Alkaline Phosphatase Blocking
Step 2 (Optional) HIER Pretreatment if needed (up to 60 mins)
Step 3 Mouse 1 Ab and Rabbit 1 Ab mixture (30-60 mins)
Step 4 Reagent 1 & Reagent 2 HRP-Polymer anti-Mouse IgG and AP- Polymer anti-Rabbit IgG require mixing (30 mins)
Step 5 Reagent 3A and Reagent 3B
Step 6 Reagent 4A, Reagent 4B& Reagent 4C Note: Make fresh working solution and use immediately. Shake Reagent 4B well before adding into Reagent 4A. GBI-Permanent Red Requires mixing! (10 min) To increase sensitivity, please repeat this step.
Step 7 Counter stain (10-15 sec)
Step 8 Reagent 5 Simpo Mount (RTU)
Results The staining pattern on the controls is correct. Y/N

IHC double staining Kits

Cat# Description Components Size
[[DS201A-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS201A-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS201A-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS201B-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS201B-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS201B-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS201C-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS201C-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS201C-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. HRP x Mouse & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS202A-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS202A-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS202A-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS202B-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS202B-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS202B-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS202C-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS202C-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS202C-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on human tissue 1. AP x Mouse & HRP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS203A-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS203A-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS203A-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS203B-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS203B-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS203B-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS203B-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS203C-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS203C-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on human tissue 1. HRP x Mouse & AP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS204A-60]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS204A-18]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS204A-6]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS204B-60]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS204B-18]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS204B-6]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS204C-60]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS204C-18]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS204C-6]] Double Staining Kits for 2 RABBIT Primary Antibodies on human or MOUSE tissue 1. HRP x Rabbit & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS205A-60]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS205A-18]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS205A-6]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. DAB (Brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS205B-60]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS205B-18]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS205B-6]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS205C-60]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS205C-18]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS205C-6]] Double Staining Kits for RABBIT and GOAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS206A-60]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS206A-18]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS206A-6]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS206B-60]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS206B-18]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS206B-6]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS206C-60]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS206C-18]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS206C-6]] Double Staining Kits for GOAT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Goat & AP x Rat
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS207A-60]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS207A-18]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS207A-6]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS207B-60]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS207B-18]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS207B-6]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS207C-60]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS207C-18]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS207C-6]] Double Staining Kits for MOUSE and GOAT Antibodies on human tissue 1. AP x Goat & HRP x Mouse
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS209A-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS209A-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS209A-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS209B-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 60 ml
[[DS209B-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 18 ml
[[DS209B-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 6 ml
[[DS209C-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS209C-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS209C-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Rat primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS210A-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS210A-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS210A-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. DAB (brown color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS210B-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 60 ml
[[DS210B-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 18 ml
[[DS210B-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. BCIP (Purple color)
4. AEC (Red color)		 6 ml
[[DS210C-60]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS210C-18]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS210C-6]] Double Staining Kits for MOUSE and RAT Primary Antibodies on human tissue 1. Mouse Primer
2. HRP x Mouse & AP x Rat
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS211A-60]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS211A-18]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS211A-6]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. DAB (brown color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS211B-60]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 60 ml
[[DS211B-18]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 18 ml
[[DS211B-6]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. BCIP (Purple color)
3. AEC (Red color)		 6 ml
[[DS211C-60]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 60 ml
[[DS211C-18]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 18 ml
[[DS211C-6]] Double Staining Kits for RABBIT and RAT Primary Antibodies on human or MOUSE tissue 1. HRP x Rat & AP x Rabbit
2. Emerald (Jade green color)
3. GBI-Permanent Red (Red color)		 6 ml
[[DS212A-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. DAB (Brown color)
4. Permanent Red (red)		 60 ml
[[DS212A-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. DAB (Brown color)
4. Permanent Red (red)		 18 ml
[[DS212A-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. DAB (Brown color)
4. Permanent Red (red)		 6 ml
[[DS212B-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. BCIP (Purple color)
4. AEC (Red color)		 60 ml
[[DS212B-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. BCIP (Purple color)
4. AEC (Red color)		 18 ml
[[DS212B-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. BCIP (Purple color)
4. AEC (Red color)		 6 ml
[[DS212C-60]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS212C-18]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS212C-6]] Double Staining Kits for 2 MOUSE Primary Antibodies on MOUSE tissue 1. Mouse Primer
2. HRP x Mouse & AP x Mouse
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS233A-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. DAB (Brown color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS233A-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. DAB (Brown color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS233A-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. DAB (Brown color)
4. GBI-Permanent Red (Red color)		 6 ml
[[DS233B-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. BCIP (Purple color)
4. AEC (Red color)		 60 ml
[[DS233B-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. BCIP (Purple color)
4. AEC (Red color)		 18 ml
[[DS233B-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. BCIP (Purple color)
4. AEC (Red color)		 6 ml
[[DS233C-60]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 60 ml
[[DS233C-18]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 18 ml
[[DS233C-6]] Double Staining Kits for MOUSE and RABBIT Primary Antibodies on MOUSE tissue 1. Mouse primer
2. HRP x Mouse & AP x Rabbit
3. Emerald (Jade green color)
4. GBI-Permanent Red (Red color)		 6 ml