AAV viral particles are generated by co-transfecting the
HEK293T cell line with a recombinant AAV vector carrying
the gene of interest (GOI), a Rep/Cap plasmid (encoding AAV Rep/Cap
genes), and an adenovirus-derived helper plasmid providing genes
needed for replication (E4, E2A, VA). OriGene offers a range of AAV vectors for your research.