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pAAV-EF1a-tGFP-WPRE AAV Gene Expression Vector

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SKU
PS100127
pAAV-AC-GFP, AAV vector with C-terminal tGFP tag and EF1a promoter
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag tGFP
E. coli Selection Ampicillin (100 ug/mL)
Expression Promoter EF1a
Features
  • OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttle, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed containing c-terminal tGFP.
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care
Gutmann, Takov, Burnap et al
Nat Commun (2021) 12 (1), 3406 DOI: 10.1038/s41467-021-23494-1
Cited by 175
AI Summary: In this study, the OriGene product pCMV6-LGALS3BP (RC204918) was used to overexpress galectin-3-binding protein (LGALS3BP) in vitro, enabling the investigation of its role in inhibiting SARS-CoV-2 spike-pseudoparticle uptake and spike-induced cell-cell fusion.
Snippet: pMD2.G (Addgene, 12259) were obtained from Addgene and pCMV6-LGALS3BP (OriGene, RC204918) from ORIGENE
FigureFigureFigure
YAP1 and COX2 coordinately regulate urothelial cancer stem-like cells
Ooki, Del Carmen Rodriguez Pena, Marchionni et al
Cancer Res. (2017)
AI Summary: In this study, OriGene’s SOX2 and YAP1 shRNA pGFP-C-shLenti Vectors, along with the non-effective 29-mer scrambled shRNA pGFP-C-shLenti Vector as a control, were used to specifically knock down SOX2 and YAP1 gene expression to investigate their roles in regulating cancer stem-like cells in urothelial carcinoma.
Snippet: SOX2 or YAP1 shRNA pGFP-C-shLenti Vector (SOX2-sh or YAP1-sh) was used for the knockdown of the gene expression (Origene). Non-effective 29-mer scrambled shRNA pGFP-C-shLenti Vector (Origene) was used as a control (SOX2- or YAP1-Ctrl)
Non-viral reprogramming of human Wharton's jelly cells reveals differences between ATOH1 homologues
Mellott, Devarajan, Shinogle et al
Tissue Eng Part A (2015)
AI Summary: OriGene's PrecisionShuttle mammalian vectors with independent turboGFP expression were used to deliver HATH1 and MATH-1 genes into human Wharton's jelly cells, enabling the study of functional differences between ATOH1 homologues in this research.
Snippet: All experiments were performed in triplicate for each cord. Plasmid and siRNA Two PrecisionShuttle mammalian vectors with independent turboGFP expression from OriGene (Rockville, MD) were used to deliver target genes to hWJCs. Cloning and verification ... Cite SaveSaving
Summary of recent deal activity
Mol Diagn (2005) 9 (4), 223-30 DOI: 10.1007/BF03260097
Showing 1-4 of 4 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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