beta Actin (ACTB) Human qPCR Primer Pair (NM_001101)

SKU
HP204660
qSTAR qPCR primer pairs against Homo sapiens gene ACTB
  $150.00
2 Weeks*
Specifications
Specifications
Product Data
Locus ID 60
Forward Sequence CACCATTGGCAATGAGCGGTTC
Reverse Sequence AGGTCTTTGCGGATGTCCACGT
ACCN NM_001101, NM_001101.1, NM_001101.2, NM_001101.3, BC013380, BC013380.2, BC001301, BC002409, BC004251, BC008633, BC009636, BC012854, BC013835, BC014401, BC014861, BC016045, BC023204, BC113036, NM_001101.5
UniProt ID P60709
Synonyms BRWS1; PS1TP5BP1
Components 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
Quality Control The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.
Storage Store at -20°C.
Stability The primer mix is stable for one year from date of shipping.
Shipping Ambient
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Documents
Resources

file-alt Citations

HIF-1α as a potential target for enhancing treatment efficacy in advanced ovarian cancer
Janc
Repository of the University of Ljubljana (2026)
AI Summary: OriGene products RPL13A (HP210356) and ACTB (HP204660) were used as reference genes for normalization in qPCR analyses to accurately measure HIF-1α expression in ovarian cancer samples in this study.
Snippet: RPL13A – F 5'CTCAAGGTGTTTGACGGCATCC3' Origene HP210356 RPL13A – R 5'TACTTCCAGCCAACCTCGTGAG3' Origene HP210356 ACTB – F 5'CACCATTGGCAATGAGCGGTC3' Origene HP204660
Oncogene activation mechanism determines the limits of targeted protein degradation
Gudauskaitė, Hernández-Morán, Taylor et al
Cell Chem Biol (2026)
AI Summary: OriGene products, specifically the Axin2 (Cat# HP207935) and β-Actin (Cat# HP204660) qPCR primer sets, were used in this study to measure gene expression levels, supporting the analysis of how oncogene activation mechanisms affect the efficacy of targeted protein degradation.
Snippet: Axin2 Forward: CAAACTTTCGCCAACCGTGGTTG origene Cat# HP207935 Axin2 Reverse: GGTGCAAAGACATAGCCAGAACC origene Cat# HP207935 β-Actin Forward: CACCATTGGCAATGAGCGGTTC origene Cat# HP204660 β-Actin Reverse: AGGTCTTTGCGGATGTCCACGT origene Cat# HP204660
A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
Lionel, Binnington, Wong et al
bioRxiv (2026)
AI Summary: OriGene's validated primer set for hACTB (Cat. #HP204660) was used to quantify gene expression in the study, aiding in the characterization of the CD4-negative HOS cell line model for HIV-1 infection and latency research.
Snippet: expression was quantified using a validated primer set (OriGene Technologies, Cat. #HP204660, Rockville, MD, USA): hACTB Forward (5'–CACCATTGGCAATGAGCGGTTC–3') and hACTB Reverse (5'–AGGTCTTTGCGGATGTCCACGT–3')
CD4 is not required for HIV-1 Infection
Lionel
Thesis (2026)
AI Summary: OriGene’s validated human ACTB (β-actin) primer set (Cat. #HP204660) was used as a normalization control in qRT-PCR assays to measure gene expression in this study investigating CD4-independent HIV-1 infection.
Snippet: … For normalization, human ACTB (β-actin) expression was quantified using a validated primer set (OriGene Technologies, Cat. #HP204660, Rockville, MD, USA): hACTB Forward (5'– CACCATTGGCAATGAGCGGTTC–3') and hACTB Reverse (5'– AGGTCTTTGCGGATGTCCACGT–3'). …
Nanoparticle-mediated targeting of PGC-1α reveals critical metabolic pathways in bladder cancer metastasis
Liu, Xin, Wu et al
Commun Biol (2025) 8 (1), 788 DOI: 10.1038/s42003-025-08174-w
Cited by 4
AI Summary: OriGene contributed to this study by providing human β-Actin primers (HP204660), which were used as an internal reference in RT-qPCR analyses to assess gene expression changes following PGC-1α silencing in bladder cancer cells.
Snippet: es. The primer sequences for RT-qPCR are shown in Table S3, and human β-Actin was used as the internal reference. The primers for β-Actin were synthesized by Wuxi AoRui Dongyuan Company (HP204660, OriGene Technologies, Wuxi, China). FISH using Poly-dT The T24 cell line was cultured under standard conditions until reaching the desired density. The cells were then divided into two groups for furt
FigureFigureFigure
Effects of miR-204-5p modulation on PAX6 regulation and corneal inflammation
Abbasi, Amini, Moustardas et al
Sci Rep (2024) 14 (1), 26436 DOI: 10.1038/s41598-024-76654-w
Cited by 23
AI Summary: OriGene's β-actin primers (human: HP204660, mouse: MP200232) were used as housekeeping reference genes in this study to normalize gene expression analyses.
Snippet: … The primer sequences used are listed in Supplementary Table 1. The sequences for β-actin primers, which are commercially available by OriGene (human: HP204660, mouse: MP200232) and widely cited as housekeeping reference genes50–52, were further validated against the human genome using NCBI’s BLAST tool to be specific with no exp
FigureFigureFigure
Showing 1-6 of 20 papers.
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