Lentiviral Control Particles

Snippet: jected into the tourniquet/IR gastrocnemius muscle in two 25 µL aliquots delivered 20 min apart by Hamilton microliter syringe. 50 μL of LV-m-Rapsyn shRNA mix (TL501861V, 1 × 107 TU/mL, OriGene Technologies, Rockville, MD) was injected into gastrocnemius muscle of sham mice. Ad-GFP (1,060, 1 × 1010 PFU/mL, VECTOR BIOLABS, Malvern, PA) or LV-shRNA scramble (TR30021V, 1 ×

Snippet: Oligodendrogliomas (ODGs) are slow-growing tumors with poorly defined biology. Here, we identify SMPD3, encoding neutral sphingomyelinase 2 (nSMase2), as a;To knock down SMPD3, BT88 and BT54 cells were transduced with four human shRNA lentiviral constructs (A–D;pGFP-c-shLenti-shSMPD3A-D, TL301492VA-D;OriGene) or scrambled shRNA control particles (pGFP-C-shLenti- shScrambled, TR30021V;OriGene)

Snippet: 2 weeks.Lentiviral delivery and stereotaxic injectionsTo knock down glycan biosynthesis, short hairpin RNA targeting PGM3 (shPGM3, TL505905V;Origene) or control shScrambled lentivirus (TR30021V;Origene) was bilaterally injected into the lateral ventricles of WT, 5xFAD and PS19 mice. Injections were performed using a stereotaxic frame at c

Snippet: thal dose for untransduced cells was selected (Supplementary Fig. 3). Commercially available lentiviral particles targeting NAALADL1 (Catalog No. TL311278V, OriGene Technologies GmbH, Herford, Germany;A-D mix in equal volumes) and corresponding control vectors (Catalog No. TR30021V, OriGene Technologies GmbH), which contain random sequences of the same l

Snippet: plasmidKIF11 human shRNA lentiviral particles (Locus ID 3832, Catalog Number: TL311921V) and their corresponding control lentiviral particles (Catalog Number: TR30021V) were obtained from Origene Inc. … The transduction of both KIF11 shRNA and control sh;- Number: TR30021V) were obtained from Origene Inc. …