VAMP2 (NM_014232) Human Over-expression Lysate
SKU
LY415423
Transient overexpression lysate of vesicle-associated membrane protein 2 (synaptobrevin 2) (VAMP2) (as WB positive control)
$436.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207533 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_014232, NP_055047 |
| Synonyms | NEDHAHM; SYB2; VAMP-2 |
| Predicted MW | 12.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_055047 |
| Locus ID | 6844 |
| Cytogenetics | 17p13.1 |
| Protein Families | WB |
| Protein Pathways | SNARE interactions in vesicular transport |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "VAMP2" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC415423 | Lyophilized VAMP2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
Evaluation of cerebrospinal fluid levels of synaptic vesicle protein, VAMP-2, across the sporadic Alzheimer's disease continuum
Goossens, Cervantes González, Dewit et al
Alzheimers Res Ther (2023) 15 (1), 186 DOI: 10.1186/s13195-023-01336-0
Goossens, Cervantes González, Dewit et al
Alzheimers Res Ther (2023) 15 (1), 186 DOI: 10.1186/s13195-023-01336-0
Cited by 19
AI Summary: OriGene’s human VAMP-2 overexpression lysate (#LY415423) was used to screen and validate monoclonal antibodies for the development of a novel digital immunoassay to measure VAMP-2 levels in cerebrospinal fluid in this study.
Snippet: eloma cells. Fused cells were plated out in 96-well plates and culture supernatants were screened on biotinylated peptide via ELISA, on HEK293T cell lysate with overexpressed human VAMP-2 (#LY415423, Origene Technologies, Rockville, USA) and on human temporal cortex extracts (Tissue Solutions, Glasgow, UK) via Western blot analysis. One monoclonal antibody (mAb), RD087, was selected and paired wit
Synaptic biomarkers in the cerebrospinal fluid associate differentially with classical neuronal biomarkers in patients with Alzheimer's disease and frontotemporal dementia
Das, Goossens, Jacobs et al
Alzheimers Res Ther (2023) 15 (1), 62 DOI: 10.1186/s13195-023-01212-x
Das, Goossens, Jacobs et al
Alzheimers Res Ther (2023) 15 (1), 62 DOI: 10.1186/s13195-023-01212-x
Cited by 60
AI Summary: OriGene’s overexpressed human VAMP2 lysate (catalog number LY415423) was used to validate antibody specificity by Western blot in the development of immunoassays for synaptic biomarkers in this study.
Snippet: e plated out in 96-well plates and culture supernatants were screened on biotinylated peptide via ELISA, and via Western Blot analysis on HEK293T cell lysate with overexpressed human VAMP2 (LY415423, Origene Technologies, Maryland, USA), and human temporal cortex extracts (Tissue Solutions, Glasgow, UK). One monoclonal antibody (mAb), 15E4, was selected and used as a detector antibody in the final
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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