DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

SKU
TA592569
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
  $519.00
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Specifications
Specifications
Product Data
Clone Name OTIR5G2
Application ELISA, FACS, FC, IF, IHC, IP, WB
Recommended Dilution WB 1:3000~10000
ELISA 1:5000
IP 1:1000
Antibody Host Rabbit
Clonality Monoclonal
Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within DDK. The exact sequence is proprietary.
Isotype IgG
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Purification Purified from cell culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Background Flag is widely used as an additional tag in expression vectors. The tag can be fused with the target protein at the N-terminal and C-terminal, and can be detected.
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Documents
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file-alt Citations

Different populations of mouse egg cortical vesicles are responsible for post-fertilization zinc sparks and proteolysis of the zona pellucida
José, Baibakov, Dean
iScience (2025) 28 (6), 112606 DOI: 10.1016/j.isci.2025.112606
Cited by 1
AI Summary: OriGene's DDK Rabbit monoclonal antibody (Cat# TA592569) was used in this study to detect proteins with N- and C-terminal tags, facilitating the analysis of cortical vesicle populations in mouse eggs.
Snippet: Rat monoclonal anti-ZP2, C-terminus Rankin et al.40 N/A Rabbit polyclonal anti-ovastacin Burkart et al.4 N/A DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags OriGene Cat# TA592569 Rabbit ZnT4 polyclonal antibody Proteintech Cat# 12623-1-AP;RRID: AB_3085398 Mouse Slc30a2 monoclonal antibody Proteintech Cat# 67993-1-lg;RRID:
PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice
Amirrad, La, Ohadi et al
Nat Commun (2025) 16 (1), 2422 DOI: 10.1038/s41467-025-57707-8
Cited by 2
AI Summary: OriGene products contributed to this study by providing the mouse PGRMC2 adeno-associated virus particles (Myc-DDK-tagged, Cat#: MR217476A1V) for ectopic expression in knockout mice and the DDK rabbit monoclonal antibody (TA592569S) for Western blot confirmation of PGRMC2 expression.
Snippet: The ectopic expression of PGRMC2 was confirmed with Western blot using a DDK rabbit monoclonal antibody (Origene, TA592569S;1:3000) and a monoclonal anti-;rescent ratiometric from the drug treatment were normalized to the baseline. Ectopic expression of PGRMC2 The mouse PGRMC2 adeno-associated virus (AAV) particles (Myc-DDK-tagged) (Cat#: MR217476A1V;Origene) were used to induce ectopic expression of PGRMC2 in the KO mice. A dose of 5 × 10¹² genome copies per KO mouse in 80 μL of saline was injected retro-orbitally into the venous sinus
FigureFigureFigure
WB
Showing 1-2 of 2 papers.
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