Tag Antibodies

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.

Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with TA592569 at 1:3000.

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with TA592569 at 1:3000.

Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.

Anti-His Immunoblot analysis of six different HEK293T over-expression lysates containing different His tagged recombinant proteins, and two his tagged bacterial lysate produced in E.coli. at 1:4000 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY YPEL3 (RC208686; Lane 1), CYSLTR1 (RC207763; Lane 2), SEMA3D (RC216032; Lane 3), Dmp1 (RC222208; Lane 4) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDK (1:4000).

Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150028).

Immunoprecipitation was performed with lysates of HEK293T cells overexpressing a GFP-tagged vector (Cat# PS100010) or DDK-tagged PON3 (Cat# LY400339). Please see datasheet for details.

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mGFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with TA592569 at 1:3000.

Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY tdTomato (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tdTomato (TA180009, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dendra2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dendra2 (TA180094, 1:500).

Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.

HEK293T cells were transfected with the pCMV6- ENTRY tdTomato cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.5ug, 1ug of lysates (lane 1 to lane 4 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit tdTomato polyclonal antibody (TA150128) at 1:10000

Black: DDK (RC212825) over-expressed HEK293T cell with TA150020 Red: DDK (RC212825) over-expressed HEK293T cell with msIgG isotype control-PE

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZsGreen1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZsGreen1 (TA180002, 1:2000).

Myc/DDK tagged VARS2 overexpression lysate and pull-down by various batches of anti-DDK beads were detected with HRP-conjugated 4C5 anti-DDK antibody (TA150030).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or HA tagged LGALS3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HA.

HEK293T cells stably expressing HIS-tagged FGFR1 clone (Cat# RC600019) screened with Ms IgG-PE (black) and Ms anti-HIS-PE (red) antibody (Cat# TA150130). Cells preparation for flow analysis was done using protocol on the OriGene website.

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY turboGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-turboGFP polyclonal antibody (TA150071) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY turboGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-turboGFP polyclonal antibody (TA150075) (1:3000).

Anti-tRFP immunoblot analysis of HEK293T over-expression lysates containing recombinant tRFP, TurboFP602 TurboFP635 fluorescence proteins at 1:2000 dilution.

Immunoprecipitation (IP) experiments were performed with negative control lysate (Cat# LY40001) generated by transfecting HEK293T cells with pCMV-entry empty vector (Cat# PS100001), and with overexpression lysate (Cat# LY400339) generated by transfecting TrueORF Gold cDNA clone myc-DDK tagged PON3 (Cat# RC210019) to HEK293T cells. Please see datasheet for details.

Human ELISA of DDK-fused protein level by using anti-DDK antibody in nuclear and cytoplasmic extracts from the peripheral blood mononuclear cells of individuals vaccinated with the BCG vaccine. Humanhttp://www.jbc.org/cgi/reprint/286/52/44799Human

Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.

CHO cells were tranfected with BAF57-Myc/DDK plasmid (OriGene Cat# RC209444). The cells were fixed with 2% formaldehyde for 20 min, and permeabilized with 0.1% saponin in PBS for 10 min. The cells were then incubated with DyLight488-labeled DDK antibodies diluted 1:50 in PBS/0.1% saponin for 20 min. The cells were then washed and observed under a fluorescent microscope. Please note the nucleus staining pattern for BAF57.

HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.2ug, 0.4ug, 0.8ug, 1.6ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit mCherry polyclonal antibody (TA150125) at 1:10000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRFP (TA180091, 1:500).

HEK293T cells were transfected with the pCMV6-ENTRY mKate (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-mKate polyclonal antibody (TA150072) (1:2000).

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

Purified N-terminal DDK fused protein (Left lane) and lysate from HEK293 cells transfected with RC209482 containing C-termnal DDK tag (right lane) were seperated with SDS-PAGE gel and immunobloted with rabbit anti-DDK tag polyclonal antibody (TA150078, 1:5000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed-Monomer (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed-Monomer.

Hela cells transiently transfected with Paxillin-GFP (RG225997) were detached and seeded onto 0.2% gelatin coated coverslip. After 24 hours incubation, the cells were fixed with 4% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with Dylight594-labeled tGFP antibodies (TA150024) and DAPI for nucleus counter staining. (a: Paxillin-GFP; b: anti-tGFP-DyLight594; c: Merged)

HEK293T cells were transfected with pCMV6-AC-GFPvector (control plasmid, Lane 1) or Myc-DDK-tagged ORF clone of human MC2R gene (Cat# RC210483, Lane 2) plasmids for 48 hrs. Eqivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myc tag-HRP. Conditio: 1:1000 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCherry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mCherry (TA180028, 1:2000).

HEK293T cells were transfected with pCMV6-AC-tGFPvector (Cat# PS100010, Lane 1) or pCMV6-Entry-MCR2 (Myc-DDK-tagged ORF clone of human MCR2 gene, Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tGFP-HRP (1:1000).

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150027).

HEK293T cells were transfected with pCMV6-ENTRY Dendra2 cDNA. 20ug cell lysate was separated by SDS-PAGE and immunoblotted with rabbit anti-Dendra2 polyclonal antibody (TA150090, 1:2000)

HEK293T cells were transfected with the pCMV6-ENTRY control vector (left lane) or pCMV6-ENTRY tGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Goat anti-tGFP polyclonal antibody (TA150096) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY YPEL3 (RC208686; Lane 1), CYSLTR1 (RC207763; Lane 2), SEMA3D (RC216032; Lane 3), Dmp1 (RC222208; Lane 4) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDK (1:4000).