Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin

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SKU
TA150015
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin
  $447.00
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Specifications
Specifications
Product Data
Clone Name OTI4C5 (formerly 4C5)
Application FC, IF, WB
Recommended Dilution WB: 1:2000, IF 1:1000, FC: 1:1000
Reactivity DDK (FLAG tag): DYKDDDDK
Antibody Host Mouse
Clonality Monoclonal
Immunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Isotype IgG2a
Formulation PBS (pH 7.4) containing 0.1% (10mg/ml) BSA, 50% glycerol and 0.02% sodium azide
Concentration 0.5mg/ml
Conjugation Biotin
Storage Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Stability Stable for 1 year at -20°C from date of shipment.
Shipping Blue Ice
Synonyms ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY, ANTI FLAG BIOTIN, ANTI DDK BIOTINK, ANTI-FLAG BIOTIN, ANTI-DDK BIOTIN
Note This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.
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file-alt Citations

AKAP79-mediated PKA-phosphorylation of STIM1 determines selective activation of ARC channels, a store-independent Orai channel
Thompson, Shuttleworth
J. Physiol. (Lond.) (2014)
Cited by 35
AI Summary: OriGene’s Flag-mAb antibody was used in this study to immunoprecipitate Flag-tagged proteins, facilitating the investigation of STIM1 phosphorylation and its role in selectively activating ARC channels.
Snippet: 16000g at 4 o C for 15 minutes). The supernatant was transferred to fresh 1.5 ml centrifuge tubes and 5 µl of a Flag- mAb (Origene, 1:200) added and rotated overnight at 4 o C. Immobilized protein A/G beads (100 µl, Pierce ... Cite SaveSaving
IP
Characterization of the genomic features and expressed fusion genes in micropapillary carcinomas of the breast
Natrajan, Wilkerson, Marchiò et al
J. Pathol. (2014)
Cited by 109
AI Summary: OriGene products, including the pCMVentry vector with a C-terminal DDK tag and full-length DDK-tagged expression constructs of FAF1, AURKA, and RAE1, were used to clone and express fusion genes and candidate genes for functional studies in breast cancer cell models in this study.
Snippet: amplified from the index tumor (MPC10) and cloned into a mammalian expression vector pCMVentry, with a C-terminal DDK tag (OriGene, MD). Full-length expression constructs of FAF1, AURKA and RAE1 with DDK tags were obtained from OriGene. Expression of ... Cite SaveSaving
Showing 1-2 of 2 papers.
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