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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

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SKU
TA50011-30
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Size:
30 ul 1 ml 100 ul 5 ml
Conjugation:
Unconjugated
  $177.00
In Stock*
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Specifications
Specifications
Product Data
Clone Name OTI4C5 (formerly 4C5)
Application FC, IF, IP, WB
Recommended Dilution WB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000
Reactivity DDK (FLAG tag): DYKDDDDK
Antibody Host Mouse
Clonality Monoclonal
Immunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Isotype IgG2a
Formulation PBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration 1 mg/ml
Conjugation Unconjugated
Storage Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Stability Stable for 1 year at -20°C from date of shipment.
Shipping Blue Ice
Synonyms ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY
Note This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.
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Other "OTI4C5 (formerly 4C5)" antibodies (14)
SKU Description Size Price
CF50011 Carrier-free (BSA/glycerol-free) Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody 100 ug
$570.00
TA150015 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin 100 ul
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TA150020 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE) 100 ul
$447.00
TA150021 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488 100 ul
$447.00
TA150022 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 550 100 ul
$447.00
TA150030 Clone OTI4C5, Anti-DDK (FLAG) Monoclonal antibody, HRP 100 ul
$447.00
TA150037 Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads 1 ml
$776.00
TA150037S Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads 500 ul
$470.00
TA150042 Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads 1 ml
$776.00
TA150042S Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads 500 ul
$470.00
TA183048 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 594 100 ul
$495.00
TA50011-1 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody 1 ml
$930.00
TA50011-100 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody 100 ul
$412.00
TA50011-5 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody 5 ml
$2,580.00

file-alt Citations

Polλ promotes microhomology-mediated end-joining
Chandramouly, Jamsen, Borisonnik et al
Nat Struct Mol Biol (2022) 30 (1), 107-114 DOI: 10.1038/s41594-022-00895-4
Cited by 29
AI Summary: OriGene's Polλ WT-Myc-DDK-tagged plasmid (catalog no. RC230157) was used to overexpress Polλ in HEK293T cells, enabling the authors to investigate Polλ's role in microhomology-mediated end-joining (MMEJ) and demonstrate its distinct activity in DNA double-strand break repair.
Snippet: The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and Save;olλ WT, 1 × 104 HEK293T cells were plated and, after 24 h, 200 ng either Polλ WT-Myc-DDK-tagged (Origene catalog no. RC230157) or Empty vector control plasmid was transfected using lipofectamine 2000. …
HIRA loss transforms FH-deficient cells
Valcarcel-Jimenez, Rogerson, Yong et al
Sci Adv (2022) 8 (42), eabq8297 DOI: 10.1126/sciadv.abq8297
Cited by 15
AI Summary: In this study, OriGene products such as the FH (OriGene #RC201307) and HIRA (OriGene #SR311083) shRNA plasmids were used to manipulate gene expression, enabling the researchers to investigate how HIRA loss affects the proliferation and transformation of FH-deficient cells.
Snippet: Fumarate hydratase (FH) is a mitochondrial enzyme that catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (TCA) cycle. Germline mutations of FH lead to hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a cancer syndrome characterized by a highly aggressive form of renal cancer. Although HLRCC tumors metastasize rapidly, FH-deficient mice develop premalignant cysts in the kidneys, rather than carcinomas. …
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Showing 1-2 of 2 papers.
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