Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads
$776.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI4C5 (formerly 4C5) |
|---|---|
| Application | IP, Protein Purification |
| Recommended Dilution | IP: See Datasheet |
| Reactivity | DDK (FLAG tag): DYKDDDDK |
| Antibody Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH |
| Isotype | IgG2a |
| Formulation | PBS (pH7.4) containing 0.1% BSA, 0.02% NaN3 and 50% glycerol with final concentration 5mg/mL beads |
| Conjugation | Magnetic Beads |
| Storage | Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. |
| Stability | Stable for 1 year at -20°C from date of shipment. |
| Shipping | Blue Ice |
| Synonyms | ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY, FLAG MAGNETIC, ANTI FLAG MAGNETIC, ANTI FLAG BEAD, ANTI FLAG RESIN, ANTI-FLAG BEAD, ANTI-FLAG RESIN, ANTI-DDK RESIN, ANTI-DDK BEAD, ANTI-DDK COLUMN, ANTI-FLAG COLUMN |
| Note | This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI4C5 (formerly 4C5)" antibodies (14)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF50011 | Carrier-free (BSA/glycerol-free) Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 100 ug |
$570.00
|
|
| TA150015 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin | 100 ul |
$447.00
|
|
| TA150020 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE) | 100 ul |
$447.00
|
|
| TA150021 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488 | 100 ul |
$447.00
|
|
| TA150022 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 550 | 100 ul |
$447.00
|
|
| TA150030 | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal antibody, HRP | 100 ul |
$447.00
|
|
| TA150037 | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads | 1 ml |
$776.00
|
|
| TA150037S | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads | 500 ul |
$470.00
|
|
| TA150042S | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads | 500 ul |
$470.00
|
|
| TA183048 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 594 | 100 ul |
$495.00
|
|
| TA50011-1 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 1 ml |
$930.00
|
|
| TA50011-100 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 100 ul |
$412.00
|
|
| TA50011-30 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 30 ul |
$177.00
|
|
| TA50011-5 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 5 ml |
$2,580.00
|
|
Citations
Lysosomal lipid peroxidation regulates tumor immunity
Bhardwaj, Lee, Versace et al
J Clin Invest (2023) 133 (8) DOI: 10.1172/JCI164596
Bhardwaj, Lee, Versace et al
J Clin Invest (2023) 133 (8) DOI: 10.1172/JCI164596
AI Summary: In this study, OriGene’s anti-DDK/Flag beads (Cat# TA150042) were used to immunoprecipitate tagged proteins, enabling the analysis of protein interactions and mechanisms underlying lysosomal lipid peroxidation-induced cell death.
Snippet: … Homogenate supernatant was then transferred to a clean 1.5 mL tube and incubated with 50 μL anti-HA beads (Pierce, 88836) or anti-DDK/Flag beads (OriGene, TA150042) for 15 minutes at 4°C. …Homogenate supernatant was then transferred to a clean 1.5mL 481 tube and incubated with 50μL anti-HA beads (Pierce Cat# 88836) or anti-DDK/Flag beads 482 (OriGene Cat# TA150042) for 15 minutes at 4°C. …
Top3α is the replicative topoisomerase in mitochondrial DNA replication
Hangas, Kekäläinen, Potter et al
Nucleic Acids Res (2022) 50 (15), 8733-8748 DOI: 10.1093/nar/gkac660
Hangas, Kekäläinen, Potter et al
Nucleic Acids Res (2022) 50 (15), 8733-8748 DOI: 10.1093/nar/gkac660
Cited by 28
AI Summary: OriGene's anti-flag magnetic beads (TA150042) were used in this study to immunoprecipitate flag-tagged proteins, enabling the investigation of Top3α's role in mitochondrial DNA replication and transcription.
Snippet: … For flag-IP, 40 μl anti-flag magnetic beads (Origene #TA150042) were added to the lysate and rotated at 4°C overnight. The beads were washed three times with RIPA buffer (10 mM Tris pH 7,4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% deoxycholate, 0;- For flag-IP, 40 μl anti-flag magnetic beads (Origene #TA150042) were added to the lysate and rotated at 4C overnight. … For flag-IP, 40 μl anti-flag magnetic beads (Origene #TA150042) were added to the lysate and rotated at 4°C overnight
CHD4 is recruited by GATA4 and NKX2-5 to repress noncardiac gene programs in the developing heart
Robbe, Shi, Wasson et al
Genes Dev (2022) 36 (7-8), 468-482 DOI: 10.1101/gad.349154.121
Robbe, Shi, Wasson et al
Genes Dev (2022) 36 (7-8), 468-482 DOI: 10.1101/gad.349154.121
Cited by 51
AI Summary: In this study, OriGene’s anti-Flag magnetic beads (TA150042) were used to perform co-immunoprecipitation experiments, enabling the authors to investigate protein interactions between CHD4 and cardiac transcription factors.
Snippet: /Myc-CHD4, TurboGFP-NKX2-5, and TurboGFP-GATA4 constructs were cotransfected into HEK293 cells, and co-IP was performed as previously reported (Waldron et al. 2016) by using anti-Flag magnetic beads (Origene TA150042) with elution buffer for 10 min at 95°C. To reduce the possibility that interacting proteins are recruited by nucleic acids, universal nucleases (Thermo Fisher 88700) were used in th
Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Rodriguez, Bailey, Larion et al
Sci Rep (2019) 9 (1), 16250 DOI: 10.1038/s41598-019-52696-3
Rodriguez, Bailey, Larion et al
Sci Rep (2019) 9 (1), 16250 DOI: 10.1038/s41598-019-52696-3
AI Summary: In this study, the DDK antibody from OriGene was used for Western blot and immunoprecipitation experiments to detect tagged proteins, contributing to the analysis of retinoid receptor turnover mechanisms in neural and glioma stem-like cells.
Snippet: sed from Sigma-Aldrich. Puromycin was from Life Sciences. Antibodies used in Western blots and immunoprecipitations included: RARA, RXRA, Sumo1, Sumo2, Ub, VCP from Santa Cruz Biotechnology, DDK from Origene, β-actin from Sigma-Aldrich, normal rabbit IgG and normal mouse IgG from Dako, and Histone H3 from Cell Signaling. WESTERN BLOT AND IMMUNOPRECIPITATION Cells were collected by scraping plat
New inhibitor targeting human transcription factor HSF1: effects on the heat shock response and tumor cell survival
Vilaboa, Boré, Martin-Saavedra et al
Nucleic Acids Res. (2017) 45 (10), 5797-5817 DOI: 10.1093/nar/gkx194
Vilaboa, Boré, Martin-Saavedra et al
Nucleic Acids Res. (2017) 45 (10), 5797-5817 DOI: 10.1093/nar/gkx194
AI Summary: OriGene’s 4C5 anti-DDK mouse monoclonal antibody-conjugated magnetic beads were used to immunoprecipitate DDK-tagged proteins from cell extracts, enabling analysis of HSF1 interactions in the study.
Snippet: Protein concentrations of extracts were determined using the protein assay reagent of Bio-Rad and were equalized prior to further analysis. Aliquots of extracts were incubated with 50 μl magnetic beads conjugated with 4C5 anti-DDK mouse monoclonal antibody (OriGene Technologies) for 2 h at 4°C with gentle agitation
Interaction of tRNA with MEK2 in pancreatic cancer cells
Wang, Chow, Ebine et al
Sci Rep (2016) 6, 28260 DOI: 10.1038/srep28260
Wang, Chow, Ebine et al
Sci Rep (2016) 6, 28260 DOI: 10.1038/srep28260
Cited by 12
AI Summary: OriGene's Anti-DDK magnetic beads (catalog no. TA150042) were used in this study to isolate and analyze MEK2 protein complexes, facilitating investigation of tRNA interactions with MEK2 in pancreatic cancer cells.
Snippet: Anti-FLAG M2 affinity gel (catalog no. A2220) was purchased from Sigma. Anti-DDK magnetic beads (catalog no. TA150042) were purchased from OriGene Technologies. FLAG peptide (catalog no. F3290) was purchased from Sigma. ... Cite SaveSaving
Showing 1-6 of 11 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
