Anti-Myc and Anti-DDK monoclonal antibodies

Snippet: DDK-tag Origene (TA150014) 1:1000 (WB) OCT3/4 Santa Cruz (sc-5279) 1:200 (WB) SOX2 Santa Cruz (sc-365823) 1:200 (WB)

Snippet: g Technology), anti-IGF-1R (#9750, Cell Signaling Technology), anti-phospho-IGF1R (#80732, Cell Signaling Technology), anti-IGF-1 antibody (#28530-1-AP. Proteintech), anti-Myc and anti-DDK (TA150014, OriGene), (Z)-4-Hydroxytamoxifen (H7904, Sigma-Aldrich), IGF-1 (I3769, Sigma-Aldrich), SAHA (SML0061, Sigma-Aldrich), DZNep (5.06069, Sigma-Aldrich), GSK1838705A (S2703, Aurogene), Everolimus (S1120,

Snippet: Affinity purified rabbit polyclonal antibody (pAb) against mouse TMC5 (epitope sequence: DFTVTHEKAVKLKQKNLSTE) was custom made by Covance. Anti-TMC4 (HPA048635) and anti-CIB3 (HPA043553) rabbit pAbs were purchased from Sigma. Anti-DDK mouse monoclonal antibodies (CAT#: TA150014) were purchased from Origene

Snippet: membranes (Bio-Rad Laboratories). Membranes were blocked in 5% milk in TBST for 1 h and then incubated with primary antibody overnight at 4°C. Primary antibodies used were: MYCN (TA150014, OriGene), SOX9 (702016, Life Technologies), PHOX2B (sc-376997, Santa Cruz Biotechnology), GAP-43

Snippet: Western-Blot The coding sequences of ICP47 N-terminus from HSV-1 (55aa, YP_009137148), HSV-2 (55aa, YP_009137225.1), and B-virus (56aa, NP_851932) were synthesized and cloned in pCMV6-Entry vector by Origene custom service. The pCMV6 vectors coding for the corresponding mutagenized sequences were synthesized and cloned as well (fig. 4B). Jurkat cells were cultured in Roswell Park Memorial Institu

Snippet: f C-terminal phosphorylation, pCMV5b-SMAD2-3SA and pCMV5b-SMAD3-3SA [38], were kindly provided by Pr. Attisano (University of Toronto, Canada). VIM-DDK and ITGB2-DDK overexpression plasmids were from Origene (Rockville, MD, USA). 2.2. Antibodies and Reagents TGFβ1 was a kind gift from Dr F.W. Ruscetti (Biological Response Modifiers Program, FRCF, NCI, Maryland, USA). All fluorophore-coupled rea