Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

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SKU
TA150039
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads
  $776.00
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Specifications
Specifications
Product Data
Clone Name OTI2H8 (formerly 2H8)
Application IP, Protein Purification
Recommended Dilution IP: See Datasheet
Reactivity tGFP
Antibody Host Mouse
Clonality Monoclonal
Immunogen Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell.
Isotype IgG2b
Formulation PBS (pH7.4) containing 0.1% BSA, 0.02% NaN3 and 50% glycerol with final concentration 5mg/mL beads
Conjugation Magnetic Beads
Storage Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Stability Stable for 1 year at -20°C from date of shipment.
Shipping Blue Ice
Predicted Protein Size 26 kDa
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file-alt Citations

AMPKα2 controls the anti-atherosclerotic effects of fish oils by modulating the SUMOylation of GPR120
Yan, Liu, Tian et al
Nat Commun (2022) 13 (1), 7721 DOI: 10.1038/s41467-022-34996-x
Cited by 15
AI Summary: In this study, the OriGene anti-tGFP antibody (TA150039) was used to detect tGFP-tagged proteins, contributing to the investigation of molecular mechanisms underlying the anti-atherosclerotic effects of fish oils.
Snippet: …A dysregulation of C-MYC leads to tumorigenesis in mouse models and is reported in almost all human cancers, which is involved in regulating a myriad of cellular processes, such as cell cycle progression, cell growth, differentiation, me;Anti-tGFP (TA150039) was purchased from OriGene (Rockville, MD). …
FigureFigureFigure
De-Palmitoylation of Tissue Factor Regulates Its Activity, Phosphorylation and Cellular Functions
Ettelaie, Featherby, Rondon et al
Cancers (Basel) (2021) 13 (15) DOI: 10.3390/cancers13153837
Cited by 8
AI Summary: In this study, OriGene products—including the human F3 (TF) cDNA clone (Cat# RC217445) and anti-TF antibody (Cat# TA500053)—were used to generate TF constructs and detect TF expression, enabling the investigation of how TF de-palmitoylation regulates its activity, phosphorylation, and cellular functions.
FigureFigureFigure
Variants identified in PTK7 associated with neural tube defects
Lei, Kim, Chen et al
Mol Genet Genomic Med (2019)
AI Summary: OriGene’s tGFP-PTK7 plasmid (CAT#: RG209690) was used in this study to express and analyze PTK7 protein variants in functional assays investigating their role in neural tube defects.
Snippet: 2.4 | Immunocytochemistry The tGFP‐PTK7 plasmid was purchased from Origene (CAT#: RG209690). PTK7 variants were introduced into tGFP‐PTK7 by site‐direct mutagenesis using GeneArt Site‐Directed Mutagenesis System (Thermo Fisher Scientific, CAT#:A14604
WB IP ICC
FOSB⁻PCDHB13 Axis Disrupts the Microtubule Network in Non-Small Cell Lung Cancer
Ting, Lee, Wu et al
Cancers (Basel) (2019) 11 (1) DOI: 10.3390/cancers11010107
AI Summary: OriGene products, specifically antibodies against Vimentin, α-Actin (smooth muscle), and PCDHB13, were used in this study to analyze protein expression and investigate the role of the FOSB⁻PCDHB13 axis in disrupting the microtubule network in non-small cell lung cancer.
Snippet: phospho-SAPK/JNK;Stathmin;α-Tubulin, clone DM1A;α/β-Tubulin;MMP-2, clone D4M2N), EMD Millipore (Calreticulin;JUND;c-JUN, clone 6A6.2;GAPDH, clone 6C5), OriGene Technologies (Rockville, MD, USA) (Vimentin;αActin (smooth muscle);PCDHB13), ABGENT (San Diego, CA, USA) (SNAIL, clone RB14258), Abcam (Cambridge, MA, USA) (Giantin), Santa Cruz Biotechnology (Santa Cruz, CA, USA) (Biotin clone 39-15D9;Prohibitin)
ICC ChIP
Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival
Losada, Muñoz-Alonso, Martínez-Díez et al
Br. J. Cancer (2018) 119 (11), 1410-1420 DOI: 10.1038/s41416-018-0336-y
AI Summary: OriGene products, including the PRDX1-Myc plasmid, anti-myc-tag and anti-tGFP-tag magnetic beads, and anti-tGFP monoclonal antibodies, were used in this study to facilitate protein detection and purification in experiments investigating the interaction between eEF1A2 and PKR in cancer cell survival.
Snippet: C16, BAY 11-7082, PF-543, and anti-FAS (CH11) antibodies (Abs) were from Merck-Millipore (Danvers, MA, USA). PRDX1-Myc plasmid, anti-myc-tag and anti-tGFP-tag magnetic beads and anti-tGFP monoclonal Abs were from Origene (Rockville, MD, USA)
IP
Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release
Ettelaie, Collier, Featherby et al
Biochim. Biophys. Acta (2016) 1863 (11), 2846-2857 DOI: 10.1016/j.bbamcr.2016.09.005
Cited by 10
Snippet: 2.2. Mutagenesis of TF plasmid and immunoprecipitation of TF protein. The pCMV-AC-TF-tGFP plasmid (OriGene, Rockville, USA) which encodes for turbo-GFP (tGFP) in tandem, on the C-terminal of TF was mutated to substitute arginine instead of Lys255. ... Cite SaveSaving
IP
Showing 1-6 of 9 papers.
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