Anti-His tag rabbit polyclonal antibody

Snippet: nalysis of successful protein transfer. After staining, the membrane was blocked for one hour in 5% skim milk dissolved in Tris-Buffered Saline 0.1% Tween 20 (TBST). Primary anti-rabbit HIS antibody (OriGene;TA150087) was applied at 1:2000 dilution and incubated overnight at 4 °C with horizontal platform shaking (62 RPM) followed by secondary goat anti-rabbit-HRP antibody (Jackson ImmunoResear

Snippet: Photosynthetic organisms harvest light using pigment-protein complexes. In cyanobacteria, these are water-soluble antennae known as phycobilisomes (PBSs). The light Save;overnight at 4°C (anti-PsbA;AS05 084A;anti-PsaB;AS10 695;anti-APC;AS08 277;Agrisera, anti-His tag;TA150087;OriGene). The membrane was washed 3 times in TBST-T

Snippet: The membrane was blocked with 5% milk in TBS (Tris 20 mM 617 and 150 mM NaCl) at room temperature for one hour then incubated with monospecific 618 polyclonal antisera in TBS-T (Tris 20 mM, 150 mM NaCl and 0.01% tween-20) overnight 619 at 4°C (anti-PsbA;AS05 084A;anti-PsaB;AS10 695;anti-APC;AS08 277;Agrisera, anti620 His tag;TA150087;OriGene). T

Snippet: 084, anti-PsaF;AS06 104, anti-SBPase;AS15 2973, anti-LHC1a;AS01 005, anti-PsaD;AS09 461, anti-Cyt f;AS08 306, anti-PaO;AS11 1783 [Agrisera], anti-GFP;PA1 980A [Invitrogen], anti-His;TA150087 [OriGene]), followed by incubation with secondary polyclonal antisera HRP (Fisher scientific, 656120) and visualized by the enhanced chemiluminescence technique (GE Healthcare). A. thaliana protoplas

Snippet: incubated overnight at 4 °C with primary antibody anti-6X His tag rabbit polyclonal antibody (Origene Technologies, Inc., Rockville, MD USA) at a dilution of 1:1000 in 5% w/v of skim milk in TBS-T (0.001% Tween) overnight at 4 °C. Unbound antibody was washed away

Snippet: Rabbit polyclonal anti-phosphor-MAP1B (Thr1265) was from Novusbio (Littleton, CO, USA). Rabbit polyclonal anti-MACF1 was from Proteintech (Chicago, IL, USA). Rabbit monoclonal anti-phosphor-GSK3β (Ser9) and mouse monoclonal anti-GSK3β were from Cell Signaling (Beverly, MA, USA). Rabbit polyclonal anti-His, mouse monoclonal anti-His and anti-GST were from Origene (Rockville, MD, USA)