Loading Control Antibody Sample Kit

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SKU
TA150046
Loading control antibody sample kit (mouse)
  $447.00
In Stock*
Bulk/Customize
Specifications
Specifications
Product Data
Application WB
Recommended Dilution WB 1:500-1:2000
Reactivity Dog, Human, Monkey, Mouse, Rat
Antibody Host Mouse
Clonality Monoclonal
Buffer TBS (pH7.4) containing0.1% BSA, 0.05% NaN3 and 50% Glycerol
Concentration 1 mg/ml
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Reference Data
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file-alt Citations

Ablation of brainstem C1 neurons improves cardiac function in volume overload heart failure
Andrade, Toledo, Díaz et al
Clin. Sci. (2019)
AI Summary: OriGene's mouse anti-HSP-90 antibody (TA150046-HSP90AB1) was used as a loading control in Western blot experiments to assess protein expression in the study investigating the role of brainstem C1 neurons in heart failure.
Snippet: Page 9. 9 were incubated at 4°C overnight with loading control antibodies diluted in the same 285 blocking solution (mouse anti- HSP-90, 1: 3000, TA150046-HSP90AB1, Origene;mouse 286 anti-GADPH, 1:2000, sc-32233, SantaCruz, for 8% and 15% gel respectively
WB
Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4
Russo, Di Benedetto, Kaganovich et al
J Neuroinflammation (2018) 15 (1), 297 DOI: 10.1186/s12974-018-1337-8
AI Summary: OriGene's mouse anti-GAPDH antibody was used in this study as a loading control for protein analysis, supporting the investigation of LRRK2's regulation of PKA signaling in microglia.
Snippet: GFP (1:20.000, Roche #11814460001), mouse anti-PKA RIIβ (1:1000, BD Biosciences #610625), mouse anti-phospho S114 PKA RIIβ (1:1000, BD Biosciences #612550), goat anti-IL-1β (1:2000, R&D system #AF401NA), and mouse anti-GAPDH (1:10.000, Origene Cached
WB
DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function
Plotegher, Berti, Ferrari et al
Sci Rep (2017) 7, 40699 DOI: 10.1038/srep40699
AI Summary: OriGene’s Loading Control Sample Kit (TA150046) was used in this study to detect housekeeping proteins (beta-tubulin and HSP90) as controls during western blot analysis, ensuring accurate assessment of alpha-synuclein levels.
Snippet: Protein was collected from the resin by adding 20 μl Laemmli buffer, loaded into 10% or 4–20% gradient SDS-PAGE and compared with the total lysate. aS detection was performed using antibodies against aS (Abcam, ab138501 for human cells and ab52168 for mouse neurons) and housekeeping proteins (beta-tubulin and HSP90) were detected using a loading control sample kit (OriGene, TA150046)
FigureFigureFigure
WB
Deciphering the role of SerpinB2 in cancer invasion and metastasis
Harris
Thesis (2017)
Snippet: …Cite SaveSaving...;20-30µg of protein from these samples were separated under non-reducing conditions by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (BioRad). The membranes were immunoprobed overnight at 4°C with antibodies against SerpinB2 (ab137588, Abcam, Cambridge, UK) and Actin (ab3280, Abcam) or GAPDH (TA150046, OriGene, Maryland, USA) as per standard protocol (refer to Supplementary Antibodies Table of Appendix)
WB
Showing 1-4 of 4 papers.
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