Anti-DDK (FLAG) rabbit polyclonal antibody

Snippet: …Primary antibodies against 135 human cMyBP-C (Santa Cruz, 137180), MyBP-HL (Barefield, 2022;1:2500) and Anti136 DDK (Origene, TA150078), phosphorylated serine 273 cMyBP-C [90], actinin 137 (ProteinTech, 14221-1-AP

Snippet: iRNA knockdown, and miRNA mimic/inhibitor experiments The expression plasmids encoding leptin (pCMV6-Flag-Lep) or a control empty vector (pCMV6-Flag) with a C-terminal Myc-DDK tag were purchased from OriGene Technologies. … Two different synthetic;- The expression plasmids encoding leptin (pCMV6-Flag-Lep) or a control empty vector (pCMV6-Flag) with a C-terminal Myc-DDK tag were purchased from OriGene Save Cached

Snippet: modelin group, xenograft tumor weight at the endpoint was also much smaller than that in the low-dose group (Figure 1G). These results suggested that the NAT10 inhibitor suppresses the growth and tumorigenesis potential of prostate cancer cells, both in vitro and in vivo. 2.2. Inhibition of NAT10 Slows DNA Replication in Prostate Cancer Cells In previous studies, the genomic depletion or chemica...

Snippet: mbrane was blocked for 2 h at room temperature in 5% powdered milk. Proteins were detected using αPilA (1:10,000) [44], αPilC (1:5,000) [37] and FLAG antibody (1:1,000) (Anti-DDK rabbit polyclonal, OriGene Technologies Inc., Rockville, MD, USA) from rabbit in a mixture with TBST (Tris-buffered saline with Tween 20;OriGene Technologies Inc., Rockville, MD, USA) in combination with a secondary an