The OriGene AAV qPCR fast Titer Kit is a specific, sensitive and accurate method to quantitate AAV titers through the measurement of AAV genome copies using SYBR green I chimeric fluorescence technology. The protocol includes an optional DNase digestion step and a short lysis step in preparation for PCR amplification. The kit is compatible with most qPCR instruments providing a convenient method to quantitate AAV in less than 2 hours.

AAV TiterNo DNA Extraction Icon

No DNA extraction step needed

AAV Titer Compatible with most qPCR Instruments

Compatible with most qPCR instrument

AAV Titer Quantitative Icon

Quantitate AAV in less than 2 hours

AAV Titer Measure all icon

Measures all serotypes

Six steps in less than 2 hours

Steps

Materials Provided in Kit

Description Quantity
2x Universal SYBR qPCR Master Mix* 1 ml
Primers Mix 100 μl
Accelerator Buffer 1.5 mL
Standard (2 x 109 GC/ml) 100 μl
UltraPure™ DNase/RNase-Free Distilled Water 1.5 mL

Available Products

SKU Description
TR30042 qPCR kit used for quantitation of AAV particles.

Example: To obtain standard equation

Standard EQ 1

Standard EQ 2

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