DDKTrap Anti-DDK Affinity Resin

SKU AR100028S

DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 1 mL

Product Data

• Applications: Affinity Chromatography, IP, Protein purification
• Background: The DDK, DYKDDDDK, or FLAG® tag is a hydrophilic short peptide specially designed for protein purification and detection, consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, abbreviated as DYKDDDDK), with a molecular weight of about 1kD. This tag is also called the DDK tag. The tag is often optimally designed and placed on the surface of a protein construct, making it convenient for purification and detection by being accessible to bind to the antibody-coated resin. OriGene DDKTrap™ Anti-DDK Affinity Resin uses anti-DDK antibodies as affinity ligands. It can be used to purify Flag-tagged fusion proteins expressed in prokaryotic, yeast, or mammalian cells in one step with high affinity. ; FLAG is a registered trademark of Sigma-Aldrich Co. LLC.
• Buffer: 50% glycerol, 1 x PBS, 0.02% NaN3
• Binding Ligand: Anti-DDK Antibody, Clone 8G6
• Matrix: 4% agarose microspheres
• Target Protein Binding Capacity: >1 mg DDK-tagged protein/ml resin
• Purity: >95% conjugated antibody purity determined by Coomassie Blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handing conditions. Avoid repeat exposures to room temperature.
• Storage: Store at -20°C immediately upon receipt.
• Shipping: Blue Ice