DDKTrap Anti-DDK Affinity Resin
Specifications
| Product Data | |
| Target Symbol | DDK |
|---|---|
| Applications | Affinity Chromatography, IP, Protein purification |
| Background | The DDK, DYKDDDDK, or FLAG® tag is a hydrophilic short peptide specially designed for protein purification and detection, consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, abbreviated as DYKDDDDK), with a molecular weight of about 1kD. This tag is also called the DDK tag. The tag is often optimally designed and placed on the surface of a protein construct, making it convenient for purification and detection by being accessible to bind to the antibody-coated resin. OriGene DDKTrap⢠Anti-DDK Affinity Resin uses anti-DDK antibodies as affinity ligands. It can be used to purify Flag-tagged fusion proteins expressed in prokaryotic, yeast, or mammalian cells in one step with high affinity.
FLAG is a registered trademark of Sigma-Aldrich Co. LLC. |
| Buffer | 50% glycerol, 1 x PBS, 0.02% NaN3 |
| Binding Ligand | Anti-DDK Antibody, Clone 8G6 |
| Matrix | 4% agarose microspheres |
| Target Protein Binding Capacity | >1 mg DDK-tagged protein/ml resin |
| Purity | >95% conjugated antibody purity determined by Coomassie Blue staining |
| Quality Control | The purity of the antibody conjugated to the resin was analyzed by SDS-PAGE analysis |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handing conditions. Avoid repeat exposures to room temperature. |
| Storage | Store at -20°C immediately upon receipt. |
| Shipping | Blue Ice |
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