DH10β Chemically Competent E. coli Cells

SKU
CC100004
DH10β Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
  $170.00
In Stock*
Specifications
Specifications
Product Data
Recommended Dilution lacZ marker for blue/white color screening
Transformation of cloned DNA into bacterial cells
Ideal for subcloning and generating cDNA libraries
Components DH10β Chemically Competent Cells – 1 mL (5 x 200 μL)
Positive Control DNA - pUC19 (50pg/μl, 25 μl )
SOC medium – 20ml
Features Chemically Competent Accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
Genotype F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-
Stability The cells are stable for 6 months from the date of shipment.
Storage The Competent Cells should be stored at -80°C. pUC19 Control DNA should be stored at -20°C and SOC recovery medium should be stored at 4°C immediately upon arrival.
Shipping Dry Ice
Description DH10β Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Documents

file-alt Citations

Deep neural networks for predicting the affinity landscape of protein-protein interactions
Moshe, Aharoni Lotati, Orenstein et al
iScience (2024)
Cited by 4
AI Summary: OriGene’s Escherichia coli DH10β bacteria were used to propagate the pPICZαA plasmid construct for protein variant purification in this study.
Snippet: The evaluation of prediction performance as a function of data characteristics is described in this and the next two subsections. For purification of the variants, the pPICZαA construct, containing the N-TIMP2 gene26 with the AOX1 promoter at its N-terminus and a hexahistidine tag at its C-terminus, and the Zeocin resistance gene, was used. The plasmid was propagated in Escherichia coli DH10β bact...
Showing 1-1 of 1 papers.
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