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PCMVMIR MicroRNA Expression Vector

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PCMVMIR
Vector for miRNA expression clone with GFP as reporter
  $670.00
In Stock*

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Specifications
Specifications
Product Data
Features
  • Express microRNA precursors from CMV promoter
  • All inserts are cloned between SgfI and MluI site
  • GFP as a reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • Can serve as control for miRNA expression experiments
Download Vector Sequence
Schematic of the multiple cloning sites
Components 1 vial of 10 ug lyophilized PCMVMIR vector
Quality Control This vector was sequence verified and restriction-digest confirmed.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
Resources

file-alt Citations

miRNA-34a-5p is a novel biomarker and modulator of the radioresponse in oesophageal adenocarcinoma
Cahill, Hackett, Kane et al
Molecular Therapy Nucleic Acids (2026) 37 (3), 103064-103064 DOI: 10.1016/j.omtn.2026.103064
AI Summary: OriGene contributed to the study by providing the miR-34a overexpression plasmid (MI0000268) and the vector control plasmid (pCMVMIR), which were used to stably overexpress miR-34a in oesophageal adenocarcinoma cells to investigate its role as a biomarker and modulator of radioresponse.
Snippet: Stable miR-34a overexpression was performed using overexpression plasmid (MI0000268) (Origene, Herford, Germany) or vector control (VC) plasmid (pCMVMIR). Cells were seeded at a density of 3 × 105 cells in a 100 mm tissue culture dish, and incubated at 37°C, 5% CO2/95% humidified air overnight
MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein
Tian, Huang, Liu et al
Oncol Lett (2024) 28 (6), 591 DOI: 10.3892/ol.2024.14724
Cited by 2
AI Summary: In this study, OriGene’s pCMV-MIR vector (cat. no. PCMVMIR), miR-24-1 precursor (cat. no. SC400296), and miR-24-2 precursor (cat. no. SC400297) were used for cotransfection experiments to assess how miR-24 regulates XIAP expression in colorectal cancer cells with different rs28382740 SNP genotypes.
Snippet: ubsequently, changes in the luciferase activity of two pMIR-REPORT vectors (pMIR-REPORT-A and pMIR-REPORT-G) in HCT116 cells were assessed by cotransfection of pCMV-MIR vectors (cat. no. PCMVMIR;OriGene Technologies, Inc.) with miR-24-1 precursor (cat. no. SC400296;OriGene Technologies, Inc.) or miR-24-2 precursor (cat. no. SC400297;OriGene Technologies, Inc.),
FigureFigureFigure
Regulation of MiR-206 in denervated and dystrophic muscles and its effect on AChR clustering
Barden, Kosloski, Jadidian et al
J Cell Sci (2024)
Cited by 6
AI Summary: OriGene’s pCMV-MIR vector (cat. no. PCMVMIR) was used to generate the miR-206-GFP plasmid construct, enabling the study of miR-206’s effects on neuromuscular junction protein expression and AChR clustering in muscle cells.
Snippet: Constructs Dtnb-GFP, Snta1-GFP, rapsyn-mCherry, and GFP-plasmid controls were generated in our lab as described in our previous work (Aittaleb et al., 2015;Valenzuela et al., 2022b). miR-206-GFP plasmid construct was generated using a pCMV-MIR vector containing GFP as a reporter (OriGene, cat. no. PCMVMIR),
Identification of miR-6794-3p as a suppressor in pancreatic cancer metastasis
Kim, Cho, Lee et al
Int J Biol Sci (2024) 20 (13), 5272-5292 DOI: 10.7150/ijbs.98490
Cited by 1
AI Summary: OriGene products, specifically the miR-Cont plasmid (#pCMVMIR) and miR-6794-3p mimic (SC402235), were used in this study to manipulate miR-6794-3p expression and investigate its role in suppressing pancreatic cancer metastasis.
Snippet: The plasmid expressing MAST1 (#VB900132-7646rqa) was purchased from Vector Builder, USA). The plasmid miR-Cont (#pCMVMIR) and miR-6794-3p mimic (SC402235) were obtained from Origene, USA. The plasmids for miR-Cont (#CmiR-AN0001-SN) and miR-6794-3p inhibitor (#HmiR-AN3685-AM02) were purchased from Gene Copoeia, USA. To construct plasmids expressing exon and intron of MAST1,
FigureFigureFigure
Hypermethylation and downregulation of microRNA219a-2 activates the ALDH1L2/GSH/PAI-1 pathway for fibronectin degradation in renal fibrosis
Wei, Xiao, Huo et al
Mol Ther (2024)
Cited by 10
AI Summary: OriGene products, specifically the pCMV-MIR empty vector and mir219a-2 overexpression plasmids, were used to manipulate microRNA-219a-2 expression in cultured renal cells, enabling the study of its role in renal fibrosis in this paper.
Snippet: … Lieberthal and J.H. Shwartz at Boston University and maintained in DMEM culture medium with 10% FBS. 54 BUMPT cells were stably transfected with empty vectors (pCMV-MIR, OriGene, Rockville, MD) or mir219a-2 overexpression plasmids (OriGene) to examine the role of mir-219a-5p in renal fibrosis. …
MicroRNA-374b regulates SARS-CoV-2 viral protein mediated endothelial to mesenchymal transition by targeting c-FLIP
Raji, Poyyakkara, Ramachandran et al
bioRxiv (2024)
Snippet: pCMVMIR vector was obtained from OriGene, USA. HiEndoXL Endothelial cell expansion medium, endothelial growth supplement, DMEM (Dulbecco's Modified Eagles
Showing 1-6 of 99 papers.
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