pMirTarget 3'-UTR Assay Vector

CAT#: PS100062

pMirTarget, the cloning vector for all 3'UTR clones for miRNA target validation, 10ug

USD 474.00


Availability*
In Stock

Size
    • 10 ug

Product images

Specifications

Product Data
E. coli Selection Kanamycin
Cell Selection Neomycin
Features

pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3’UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity.

  • High sensitivity of Luciferase assay due to IRES-driven luciferase cassette
  • RFP as a reporter for transfection monitoring and normalization
  • Neomycin selection marker for stable cell establishment
  • Compatible with Precision shuttling system
  • Can serve as a control for target validation experiments.
  • 3-UTR Clones for MicroRNA target validation
  • Download full vector sequence
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (23)

Documents