Cas9 Protein

Cas9 protein is an RNA-guided endonuclease that can be used for the site-specific cleavage of double stranded DNA. OriGene offers a complete set of Cas9 products from DNA clone to purified protein and specific antibody. All of them are created based on wild type Streptococcus pyogenes Cas9 protein.

Why Choose OriGene Cas9 Protein?

  • Sterile protein with NLS signals
  • High RNA-guided endonuclease activity
  • High genome editing efficiency
  • High protein concentration & purity
Cas9 protein

Featured products:


SKU Description Manual Size Availability
TP790148 CAS9 S. pyogenes Recombinant Protein 500 pmol In stock
TP790151 CAS9-D10A S. pyogenes Recombinant Protein 500 pmol In stock

Functional studies of wild type Cas9 protein (Cat. No. TP790148)

In vitro cleavage View Protocol

In vitro cleavage

Figure 1: In-vitro digestion of PS100074 vector with purified Cas9 protein (Cat. No. TP790148) and specific sgRNA. Purified Cas9 protein can cut the substrate DNA only when it is incubated with specific sgRNA.

In vivo genome editing View Protocol

In vivo genome editing

Figure 2: In-vivo genome editing of tRFP positive cells with purified Cas9 protein (Cat. No. TP790148) and specific sgRNA. Purified Cas9 protein can knockout the expression of tRFP protein in the tRFP postive cells when co-transefected with specific sgRNA.

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