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pCas-Guide-CRISPRa gene activation vector

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SKU
GE100055
pCas-Guide-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with dCas9-VP64 and gRNA cloning site.
  $670.00
In Stock*

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Specifications
Specifications
Product Data
Function All-in-one CRISPRa SAM vector
E. coli Selection Ampicillin (100 ug/mL)
Features
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • gRNA under U6 promoter
  • gRNA is modified with MS2 RNA aptamer in the loops
  • CMV driven dCas9-VP64, which can activate gene expression
  • For robust gene activation, needs to work with pCRISPRa-Enhancer
  • CF3 primer can be used to sequence target sequence after cloning
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Assessment of CRISPRa-mediated gdnf overexpression in an In vitro Parkinson's disease model
Guzmán-Sastoque, Sotelo, Esmeral et al
Front Bioeng Biotechnol (2024) 12, 1420183 DOI: 10.3389/fbioe.2024.1420183
Cited by 15
AI Summary: OriGene products pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were used to enable CRISPRa-mediated overexpression of gdnf in the in vitro Parkinson's disease model described in the study.
Snippet: 0S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Biolabs (Ipswich, MA, United States). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were obtained from Origene (Rockville, MD, United States). The isolation of the primary culture of rat astrocytes, microglia, and neurons will be described in the methodology section 2.9. 2.2 MNP synthesis The synthesi
FigureFigureFigure
Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing Conditions
Arango, Cifuentes, Puentes et al
Nanomaterials (Basel) (2023) 13 (11) DOI: 10.3390/nano13111782
Cited by 13
AI Summary: OriGene products pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were used as CRISPR activation vectors in this study to demonstrate the gene delivery and overexpression capabilities of the developed magnetite-nanoparticle-based nanocarriers.
Snippet: (NEB-T1030S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Bio labs (Ipswich, MA, USA). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were obtained from Origene (Rockville, MD, USA). Cell characterization was conducted in human primary dermal fibroblasts (HDFa, PCS-201-012) purchased from ATCC (St Cloud, MN, USA), and human astrocytes (NHA) were obtai
FigureFigureFigure
Showing 1-2 of 2 papers.
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