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pAAVS1-RFP-DNR

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SKU
GE100026
Positive control for AAVS1 insertion (RFP insertion via CRISPR)
  $670.00
In Stock*

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Specifications
Specifications
Product Data
Function AAVS1 donor vector
Features
  • AAVS1 donor vector containing PGK-puro and CMV-RFP
  • CRISPR knockin positive control when paired with pCas-guide-AAVS1
  • Contains AAVS1 left and right homologous sequences (550 bp each side)
  • PGK-puro and CMV-RFP cassette can be integrated into human AAVS1 site when cotransfected with pCas-guide-AAVS1
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Hyperforin/HP-β-Cyclodextrin Enhances Mechanosensitive Ca(2+) Signaling in HaCaT Keratinocytes and in Atopic Skin Ex Vivo Which Accelerates Wound Healing
Takada, Yonekawa, Matsumoto et al
Biomed Res Int (2017) 2017, 8701801 DOI: 10.1155/2017/8701801
AI Summary: OriGene’s TRPC6 shRNA plasmid (TF308626) was used in this study to knock down TRPC6 expression in HaCaT keratinocytes, enabling the researchers to investigate the role of TRPC6 in hyperforin/HP-β-cyclodextrin-enhanced Ca(2+) signaling and wound healing.
Snippet: 2.7. The Knockdown of TRPC6 by shRNA. TRPC6 shRNA plasmids that coexpressed RFP (TF308626;OriGene Technologies, Rockville, MD) were used. The shTRPC6 targeting sequence was 59-AAGCAGGACATCTCAAGTCTCCGCTATGA-39. ... All 7 versions Cite SaveSaving
FigureFigureFigure
Tumor-derived inducible heat-shock protein 70 (HSP70) is an essential component of anti-tumor immunity
Dodd, Nance, Quezada et al
Oncogene (2014)
Cited by 32
AI Summary: OriGene’s HuSH pRFP-c-RS shRNAs targeting Hspa1a and Hspa1b, as well as the pRFP-c-RS negative control shRNA, were used to stably knock down HSP70 expression in wild-type transformants, enabling the study of HSP70’s role in anti-tumor immunity.
Snippet: Plasmids used/transfections/transductions. WT transformants stably expressing HuSH pRFP-c-RS shRNAs for Hspa1a and Hspa1b or the shRNA pRFP-c-RS negative control (all purchased from OriGene, Rockville, MD, USA) were generated. ... Cite SaveSaving
Constitutive clathrin-mediated endocytosis of CTLA-4 persists during T cell activation
Qureshi, Kaur, Hou et al
J. Biol. Chem. (2012) 287 (12), 9429-40 DOI: 10.1074/jbc.M111.304329
Cited by 8
AI Summary: OriGene’s shRNA targeting AP-2 (co-expressing RFP) was used in this study to investigate the role of clathrin-mediated endocytosis in CTLA-4 trafficking during T cell activation.
Snippet: shRNA targeting AP-2 and co-expressing RFP were purchased from Origene. ... CTLA-4 has been reported to be delivered to the cell surface from secretory lysosomes;however, its relatively short half-life makes it questionable whether this would be a storage compartment of the
Showing 1-3 of 3 papers.
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